Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta (DE3) cells | Streptomyces lividans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 4.94% relative activity at 2 mM | Streptomyces lividans | |
Co2+ | 46.47% relative activity at 2 mM | Streptomyces lividans | |
Cu2+ | complete inhibition at 2 mM | Streptomyces lividans | |
EDTA | complete inhibition at 2 mM | Streptomyces lividans | |
K+ | 3.53% relative activity at 2 mM | Streptomyces lividans | |
Na+ | 4.67% relative activity at 2 mM | Streptomyces lividans | |
Zn2+ | 1.85% relative activity at 2 mM | Streptomyces lividans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00242 | - |
NADP+ | in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+ | Streptomyces lividans | |
0.00278 | - |
NADP+ | in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+ | Streptomyces lividans | |
8.49 | - |
NAD+ | in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+ | Streptomyces lividans | |
18.6 | - |
NAD+ | in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+ | Streptomyces lividans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the recombinant IDH displays a 85000fold greater specificity for NADP+ than NAD+ with Mg2+ | Streptomyces lividans | |
Mn2+ | the recombinant IDH displays a 62000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+ | Streptomyces lividans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
gel filtration | Streptomyces lividans |
80000 | - |
SDS-PAGE | Streptomyces lividans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lividans | B2ZAA4 | - |
- |
Streptomyces lividans TK54 | B2ZAA4 | - |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Streptomyces lividans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-isocitrate + NAD+ | slow reaction | Streptomyces lividans | 2-oxoglutarate + CO2 + NADH | - |
? | |
(2R,3S)-isocitrate + NAD+ | slow reaction | Streptomyces lividans TK54 | 2-oxoglutarate + CO2 + NADH | - |
? | |
(2R,3S)-isocitrate + NADP+ | - |
Streptomyces lividans | 2-oxoglutarate + CO2 + NADPH | - |
? | |
(2R,3S)-isocitrate + NADP+ | - |
Streptomyces lividans TK54 | 2-oxoglutarate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 72000, gel filtration | Streptomyces lividans |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Streptomyces lividans |
NADP+-dependent IDH | - |
Streptomyces lividans |
NADP-IDH | - |
Streptomyces lividans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
around 46°C | Streptomyces lividans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | 50 | about 50% of IDH activity is preserved at 38°C after 20 min of incubation, the enzyme is inactive at 50°C | Streptomyces lividans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.37 | - |
NAD+ | in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+ | Streptomyces lividans | |
2.11 | - |
NAD+ | in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+ | Streptomyces lividans | |
23.2 | - |
NADP+ | in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mg2+ | Streptomyces lividans | |
27.6 | - |
NADP+ | in 20 mM Tris-HCl (pH 8.0), at 25°C, in the presence of 2 mM Mn2+ | Streptomyces lividans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
in the presence of 2 mM Mn2+ | Streptomyces lividans |
9 | - |
in the presence of 2 mM Mg2+ | Streptomyces lividans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the recombinant IDH displays a 62000fold (kcat/Km) preference for NADP+ over NAD+ with Mn2+, and a 85000fold greater specificity for NADP+ than NAD+ with Mg2+ | Streptomyces lividans |