Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | 50% inhibition of the forward reaction at 1.5 mM, 99% inhibition at 15 mM | Chlorobium limicola | |
2-oxoglutarate | 50% inhibition of the forward reaction at 1.5 mM, 95% inhibition at 15 mM | Rhodopseudomonas palustris | |
isocitrate | 50% inhibition of the reverse reaction at 0.1 mM, 97% inhibition at 1.2 mM | Chlorobium limicola | |
isocitrate | 70% inhibition of the reverse reaction at 0.1 mM, 99% inhibition at 1.2 mM | Rhodopseudomonas palustris | |
additional information | no inhibition by glyoxylate | Chlorobium limicola | |
additional information | no inhibition by glyoxylate | Rhodopseudomonas palustris | |
NADP+ | 37% inhibition of the reverse reaction at 4.0 mM | Chlorobium limicola | |
NADP+ | 35% inhibition of the reverse reaction at 4.0 mM | Rhodopseudomonas palustris | |
NADPH | 42% inhibition of the forward reaction at 0.5 mM | Chlorobium limicola | |
NADPH | 66% inhibition of the forward reaction at 0.5 mM | Rhodopseudomonas palustris | |
NaHCO3 | 28% inhibition of the forward reaction at 10 mM | Chlorobium limicola | |
NaHCO3 | 9% inhibition of the forward reaction at 10 mM | Rhodopseudomonas palustris | |
oxaloacetate | 18.6% inhibition at 2.5 mM | Chlorobium limicola | |
oxaloacetate | 15.5% inhibition at 2.5 mM | Rhodopseudomonas palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
isocitrate | pH 8.0, forward reaction | Chlorobium limicola | |
0.007 | - |
NADP+ | pH 8.0, forward reaction | Chlorobium limicola | |
0.009 | - |
isocitrate | pH 8.0, forward reaction | Rhodopseudomonas palustris | |
0.01 | - |
NADPH | pH 6.5, reverse reaction | Chlorobium limicola | |
0.017 | - |
NADP+ | pH 8.0, forward reaction | Rhodopseudomonas palustris | |
0.034 | - |
NADPH | pH 6.5, reverse reaction | Rhodopseudomonas palustris | |
0.304 | - |
2-oxoglutarate | pH 6.5, reverse reaction | Chlorobium limicola | |
0.398 | - |
2-oxoglutarate | pH 6.5, reverse reaction | Rhodopseudomonas palustris | |
11.68 | - |
CO2 | pH 6.5, reverse reaction | Chlorobium limicola | |
13.82 | - |
CO2 | pH 6.5, reverse reaction | Rhodopseudomonas palustris |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Chlorobium limicola | 5829 | - |
cytosol | - |
Rhodopseudomonas palustris | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | divalent cation is required, optimal activity at 2 mM in the forward reaction, and at 200 mM in the reverse reaction | Rhodopseudomonas palustris | |
Mg2+ | divalent cation is required, optimal activity at 40 mM in the forward reaction, and at 250 mM in the reverse reaction | Chlorobium limicola | |
Mn2+ | divalent cation is required, optimal activity at 1 mM in the forward reaction, and at 20 mM in the reverse reaction | Rhodopseudomonas palustris | |
Mn2+ | divalent cation is required, optimal activity at 10 mM in the forward reaction, and at 50 mM in the reverse reaction | Chlorobium limicola |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64400 | - |
gel filtration | Chlorobium limicola |
69100 | - |
gel filtration | Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Chlorobium limicola | metabolic regulation, involved in the tricarboxylic acid cycle | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | Rhodopseudomonas palustris | metabolic regulation, involved in the tricarboxylic acid cycle | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | Rhodopseudomonas palustris 285 | metabolic regulation, involved in the tricarboxylic acid cycle | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobium limicola | - |
formerly Chlorobium thiosulfatophilum, strain L | - |
Rhodopseudomonas palustris | - |
- |
- |
Rhodopseudomonas palustris 285 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
41fold by ultracentrifugation, ammonium sulfate fractionation, gel filtration and ion exchange chromatography, and desalting | Chlorobium limicola |
by ultracentrifugation, ammonium sulfate fractionation, gel filtration and ion exchange chromatography, and desalting | Rhodopseudomonas palustris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.04 | - |
purified enzyme, reverse reaction | Chlorobium limicola |
0.28 | - |
purified enzyme, forward reaction | Chlorobium limicola |
Storage Stability | Organism |
---|---|
-18°C, purified enzyme, 50% glycerol, stable for at least 1.5-2 months | Chlorobium limicola |
-18°C, purified enzyme, 50% glycerol, stable for at least 1.5-2 months | Rhodopseudomonas palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + CO2 + NADPH | - |
Chlorobium limicola | isocitrate + NADP+ | - |
r | |
2-oxoglutarate + CO2 + NADPH | - |
Rhodopseudomonas palustris | isocitrate + NADP+ | - |
r | |
2-oxoglutarate + CO2 + NADPH | - |
Rhodopseudomonas palustris 285 | isocitrate + NADP+ | - |
r | |
isocitrate + NADP+ | - |
Chlorobium limicola | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | - |
Rhodopseudomonas palustris | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | metabolic regulation, involved in the tricarboxylic acid cycle | Chlorobium limicola | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | metabolic regulation, involved in the tricarboxylic acid cycle | Rhodopseudomonas palustris | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | - |
Rhodopseudomonas palustris 285 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | metabolic regulation, involved in the tricarboxylic acid cycle | Rhodopseudomonas palustris 285 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
ICDH | - |
Chlorobium limicola |
ICDH | - |
Rhodopseudomonas palustris |
isocitrate dehydrogenase | - |
Chlorobium limicola |
isocitrate dehydrogenase | - |
Rhodopseudomonas palustris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
5 min, complete loss of activity | Chlorobium limicola |
50 | - |
5 min, complete loss of activity | Rhodopseudomonas palustris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
reverse reaction | Rhodopseudomonas palustris |
6.7 | - |
reverse reaction | Chlorobium limicola |
8.1 | - |
forward reaction | Chlorobium limicola |
8.3 | - |
forward reaction | Rhodopseudomonas palustris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Chlorobium limicola | |
NADP+ | - |
Rhodopseudomonas palustris | |
NADPH | - |
Chlorobium limicola | |
NADPH | - |
Rhodopseudomonas palustris |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodopseudomonas palustris | isoelectric focusing | - |
4.9 |
Chlorobium limicola | isoelectric focusing | - |
6 |