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Literature summary for 1.1.1.42 extracted from
- Cytosolic NADP+-dependent isocitrate dehydrogenase plays a key role in lipid metabolism (2004), J. Biol. Chem., 279, 39968-39974.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Mus musculus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the cDNA in a construct of nucleotides 1-1714 with rat PEPCK promotor and the SV-40 polyadenylation signal, permanant expression in 3T3-L1 cells and in transgenic mice, the latter are constructed by infection of fertilized eggs | Mus musculus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of transgenic mice by infection of fertilized eggs, enzyme overexpressing transgenic mice show increased triglyceride and cholesterol levels and develop fatty liver, hyperlipidemia, and obesity | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mus musculus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | Mus musculus | cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
26-weeks-old male mice | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | - |
Mus musculus | - |
| adipose tissue | - |
Mus musculus | - |
| epididymis | epididymal fat pad | Mus musculus | - |
| fat pad | epididymal fat pad, hepatic fat pad | Mus musculus | - |
| liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.6 | - |
wild-type epidydymal fat pad | Mus musculus |
| 50 | - |
transgenic mice epidydymal fat pad | Mus musculus |
| 77.4 | - |
wild-type liver | Mus musculus |
| 108.2 | - |
transgenic mice liver | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells | Mus musculus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
| threo-Ds-isocitrate + NADP+ | - |
Mus musculus | 2-oxoglutarate + CO2 + NADPH | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IDPc | - |
Mus musculus |
| NADP+-dependent isocitrate dehydrogenase | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | essential | Mus musculus | |
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Release 2023.1 (January 2023)
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