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Literature summary for 1.1.1.42 extracted from
- NADP-dependent isocitrate dehydrogenase from the halophilic archaeon Haloferax volcanii: cloning, sequence determination and overexpression in Escherichia coli (2002), FEMS Microbiol. Lett., 209, 155-160.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| IDCH gene, DNA and amino acid sequence determination by combinated PCR and screening of genomic lambda EMBL3 library, and sequence analysis, overexpression in Escherichia coli BL21(DE3) as insoluble protein in inclusion bodies | Haloferax volcanii |
General Stability
| General Stability | Organism |
|---|---|
| 3 M NaCl stabilizes the recombinant enzyme | Haloferax volcanii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.093 | - |
NADP+ | recombinant, renatured enzyme, pH 8.0 | Haloferax volcanii |  |
| 0.105 | - |
DL-isocitrate | recombinant, renatured enzyme, pH 8.0 | Haloferax volcanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Haloferax volcanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45837 | - |
x * 45837, sequence calculation, x * 60000, recombinant, renatured enzyme, SDS-PAGE | Haloferax volcanii |
| 60000 | - |
x * 45837, sequence calculation, x * 60000, recombinant, renatured enzyme, SDS-PAGE | Haloferax volcanii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax volcanii | Q8X277 | halophilic archaeon | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant solubilized enzyme by ultrafiltration, and DEAE ion exchange chromatography, 6.4fold | Haloferax volcanii |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| solubilization in presence of 3 M NaCl of recombinant enzyme from inclusion bodies after expression in Escherichia coli | Haloferax volcanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 23.7 | - |
purified recombinant enzyme | Haloferax volcanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DL-isocitrate + NADP+ | - |
Haloferax volcanii | 2-oxoglutarate + CO2 + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45837, sequence calculation, x * 60000, recombinant, renatured enzyme, SDS-PAGE | Haloferax volcanii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ICDH | - |
Haloferax volcanii |
| NADP+-dependent Ds-threo-isocitrate dehydrogenase | - |
Haloferax volcanii |
| NADP+-dependent Ds-threo-isocitrate:NADP+ oxidoreductase | - |
Haloferax volcanii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
recombinant enzyme, 93% remaining activity after 117 h, in presence of 3 M NaCl | Haloferax volcanii |
| 80 | - |
recombinant enzyme, inactivation within 2.5 min, in presence of 3 M NaCl | Haloferax volcanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Haloferax volcanii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Haloferax volcanii | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Haloferax volcanii | calculated | - |
4.9 |
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