Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the mitochondrial isozyme is light-inducible, while the cytosolic one is not | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Pisum sativum | |
isocitrate | reverse reaction | Pisum sativum | |
additional information | poor inhibition of the forward reaction by 2-oxoglutarate, no inhibition by CO2 | Pisum sativum | |
NADH | noncompetitive | Pisum sativum | |
NADP+ | reverse reaction | Pisum sativum | |
NADPH | forward reaction | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
NADP+ | mitochondrial isozyme, forward reaction, pH 8.0 | Pisum sativum | |
0.004 | - |
NADP+ | cytosolic isozyme, forward reaction, pH 8.0 | Pisum sativum | |
0.004 | - |
isocitrate | mitochondrial isozyme, forward reaction, pH 8.0 | Pisum sativum | |
0.006 | - |
isocitrate | cytosolic isozyme, forward reaction, pH 8.0 | Pisum sativum | |
0.009 | - |
NADPH | mitochondrial isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
0.017 | - |
2-oxoglutarate | mitochondrial isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
0.024 | - |
NADPH | cytosolic isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
0.026 | - |
2-oxoglutarate | cytosolic isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
0.2 | - |
CO2 | mitochondrial isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
1.6 | - |
CO2 | cytosolic isozyme, reverse reaction, pH 7.0 | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pisum sativum | 5829 | - |
mitochondrion | light-inducible isozyme | Pisum sativum | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Pisum sativum | the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
cv. 294 040 Märgärt Kelvedon Wonder, one cytosolic and one mitochondrial isozyme | - |
Purification (Comment) | Organism |
---|---|
cytosolic and mitochondrial isozymes | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | green and etiolated | Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.038 | - |
mitochondria from etiolated leaves, forward reaction at pH 7.5 | Pisum sativum |
0.072 | - |
mitochondria from green leaves, forward reaction at pH 7.5 | Pisum sativum |
Storage Stability | Organism |
---|---|
-80°C, partially purified cytosolic and mitochondrial isozymes, several months, stable | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Pisum sativum | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
isocitrate + NADP+ | the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview | Pisum sativum | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADP-dependent isocitrate dehydrogenase | - |
Pisum sativum |
NADP-ICDH | - |
Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.3 | 6.5 | reverse reaction, cytosolic isozyme | Pisum sativum |
6.8 | 7 | reverse reaction, mitochondrial isozyme | Pisum sativum |
8 | 9 | forward reaction, cytosolic and mitochondrial isozymes | Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NAD+ and NADH | Pisum sativum | |
NADP+ | absolutely specific for, forward reaction | Pisum sativum | |
NADPH | absolutely specific for, reverse reaction | Pisum sativum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
NADPH | mitochondrial isozyme, forward reaction, pH 8.0 | Pisum sativum | |
0.005 | - |
isocitrate | mitochondrial isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
0.006 | - |
NADP+ | mitochondrial isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
0.007 | - |
NADPH | cytosolic isozyme, forward reaction, pH 8.0 | Pisum sativum | |
0.012 | - |
isocitrate | cytosolic isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
0.021 | - |
NADP+ | cytosolic isozyme, reverse reaction, pH 7.0 | Pisum sativum | |
0.06 | - |
NADH | mitochondrial isozyme, forward reaction, pH 8.0 | Pisum sativum | |
0.08 | - |
NADH | cytosolic isozyme, forward reaction, pH 8.0 | Pisum sativum | |
1 | - |
2-oxoglutarate | above, cytosolic isozyme, forward reaction, pH 8.0 | Pisum sativum | |
1 | - |
2-oxoglutarate | above, mitochondrial isozyme, forward reaction, pH 8.0 | Pisum sativum |