Literature summary for 1.1.1.42 extracted from

  • Dhariwal, K.R.; Venkitasubramanian, T.A.
    NADP-specific isocitrate dehydrogenase of Mycobacterium phlei ATCC 354: purification and characterization (1987), J. Gen. Microbiol., 133, 2457-2460.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Mycolicibacterium phlei
dithiothreitol
-
Mycolicibacterium phlei
glutathione
-
Mycolicibacterium phlei
glyoxylate plus oxaloacetate Mycolicibacterium phlei
Hg2+
-
Mycolicibacterium phlei
monoiodoacetate
-
Mycolicibacterium phlei
Oxalomalate
-
Mycolicibacterium phlei
p-chloromercuribenzoate
-
Mycolicibacterium phlei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
NADP+
-
Mycolicibacterium phlei
0.074
-
D,L-isocitrate
-
Mycolicibacterium phlei

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ absolute requirement for divalent cations Crithidia fasciculata
Mg2+ absolute requirement for divalent cations Mycolicibacterium phlei
Mg2+ absolute requirement for divalent cations [Brevibacterium] flavum
Mg2+ absolute requirement for divalent cations Starkeya novella
Mn2+ absolute requirement for divalent cations Crithidia fasciculata
Mn2+ absolute requirement for divalent cations Mycolicibacterium phlei
Mn2+ absolute requirement for divalent cations [Brevibacterium] flavum
Mn2+ absolute requirement for divalent cations Starkeya novella
Zn2+ absolute requirement for divalent cations Mycolicibacterium phlei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Crithidia fasciculata
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Mycolicibacterium phlei
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ [Brevibacterium] flavum
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Starkeya novella
-
2-oxoglutarate + NADPH + H+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
[Brevibacterium] flavum
-
-
-
Crithidia fasciculata
-
-
-
Mycolicibacterium phlei
-
-
-
Starkeya novella
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycolicibacterium phlei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
109
-
-
Mycolicibacterium phlei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Crithidia fasciculata 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Mycolicibacterium phlei 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
[Brevibacterium] flavum 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Starkeya novella 2-oxoglutarate + NADPH + H+ + CO2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 30
-
Mycolicibacterium phlei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Mycolicibacterium phlei

pH Range

pH Minimum pH Maximum Comment Organism
6.5 10
-
Mycolicibacterium phlei

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Crithidia fasciculata
NADP+
-
Mycolicibacterium phlei
NADP+
-
[Brevibacterium] flavum
NADP+
-
Starkeya novella