Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.42 extracted from

  • Ramaley, R.F.; Hudock, M.O.
    Purification and properties of isocitrate dehydrogenase (NADP) from Thermus aquaticus YT-1, Bacillus subtilis-168 and Chlamydomonas reinhardti-Y-2 (1973), Biochim. Biophys. Acta, 315, 22-36.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
glyoxylate plus oxaloacetate Bacillus subtilis
glyoxylate plus oxaloacetate Chlamydomonas reinhardtii
glyoxylate plus oxaloacetate Thermus aquaticus
Oxalomalate
-
Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0091
-
D,L-isocitrate
-
Bacillus subtilis
0.0097
-
D,L-isocitrate 25°C assay temperature Thermus aquaticus
0.01
-
NADP+
-
Chlamydomonas reinhardtii
0.0125
-
D,L-isocitrate
-
Chlamydomonas reinhardtii
0.0165
-
NADP+ 25°C assay temperature Thermus aquaticus
0.0181
-
D,L-isocitrate 70°C assay temperature Thermus aquaticus
0.0274
-
NADP+
-
Bacillus subtilis
0.031
-
NADP+ 70°C assay temperature Thermus aquaticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000 70000 gel filtration Thermus aquaticus
75000 80000 gel filtration Bacillus subtilis
80000
-
gel filtration Escherichia coli
80000
-
gel filtration Azotobacter vinelandii
87000 92600 gel filtration Geobacillus stearothermophilus
90000 100000 gel filtration Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Thermus aquaticus
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Chlamydomonas reinhardtii
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Bacillus subtilis
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Escherichia coli
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Geobacillus stearothermophilus
-
2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+ Azotobacter vinelandii
-
2-oxoglutarate + NADPH + H+ + CO2
-
?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Bacillus subtilis
-
-
-
Chlamydomonas reinhardtii
-
-
-
Escherichia coli
-
-
-
Geobacillus stearothermophilus
-
-
-
Thermus aquaticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus aquaticus
-
Chlamydomonas reinhardtii
-
Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Thermus aquaticus 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Chlamydomonas reinhardtii 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Bacillus subtilis 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Escherichia coli 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Geobacillus stearothermophilus 2-oxoglutarate + NADPH + H+ + CO2
-
?
isocitrate + NADP+
-
Azotobacter vinelandii 2-oxoglutarate + NADPH + H+ + CO2
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay condition Thermus aquaticus
25
-
assay condition Chlamydomonas reinhardtii
25
-
assay condition Bacillus subtilis
25
-
assay condition Geobacillus stearothermophilus
37
-
-
Bacillus subtilis
70
-
-
Thermus aquaticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
50% loss of activity in 1 h Chlamydomonas reinhardtii
54
-
50% loss of activity in 1 h Bacillus subtilis
88
-
50% loss of activity in 1 h Thermus aquaticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9
-
Thermus aquaticus