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Literature summary for 1.1.1.42 extracted from
- Isocitrate dehydrogenase (1963), The Enzymes, 2nd Ed. (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 7, 105-126.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| instable in solutions of low ionic strength, stabilization by ammonium sulfate | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | decarboxylation of isocitrate and oxalosuccinate | Sus scrofa |  |
| Cu2+ | - |
Sus scrofa | |
| Diphenylchloroarsine | - |
Sus scrofa | |
| isocitrate | competitive inhibition of decarboxylation of oxalosuccinate | Sus scrofa | |
| p-chloromercuribenzoate | - |
Sus scrofa | |
| p-hydroxymercuribenzoate | decarboxylation of isocitrate and oxalosuccinate | Sus scrofa | |
| Phenarsazines | - |
Sus scrofa | |
| Phenylmercuric nitrate | - |
Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0026 | - |
D,L-isocitrate | - |
Sus scrofa | |
| 0.0092 | - |
NADPH | - |
Sus scrofa | |
| 0.13 | - |
2-oxoglutarate | - |
Sus scrofa | |
| 0.56 | - |
oxalosuccinate | reductase | Sus scrofa | |
| 1.2 | - |
oxalosuccinate | decarboxylase | Sus scrofa | |
| 25 | 26 | oxalosuccinate | decarboxylase | Sus scrofa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement for divalent cations | Sus scrofa | |
| Mg2+ | absolute requirement for divalent cations | Aspergillus niger | |
| Mn2+ | absolute requirement for divalent cations | Sus scrofa | |
| Mn2+ | absolute requirement for divalent cations | Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | 64000 | ultracentrifugation | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | Sus scrofa | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
r | |
| isocitrate + NADP+ | Saccharomyces cerevisiae | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | Aspergillus niger | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | Mycobacterium tuberculosis | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | Trypanosoma cruzi | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | Citrobacter freundii | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Citrobacter freundii | - |
- |
- |
| Mycobacterium tuberculosis | - |
- |
- |
| Saccharomyces cerevisiae | - |
- |
- |
| Sus scrofa | - |
pig | - |
| Trypanosoma cruzi | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NADP+ | - |
Sus scrofa | 2-oxoglutarate + NADPH + H+ + CO2 | - |
r | |
| isocitrate + NADP+ | - |
Saccharomyces cerevisiae | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | - |
Aspergillus niger | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | - |
Mycobacterium tuberculosis | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | - |
Trypanosoma cruzi | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| isocitrate + NADP+ | - |
Citrobacter freundii | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
| oxalosuccinate | - |
Sus scrofa | 2-oxoglutarate + CO2 | - |
r | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 58.3 | - |
isocitrate | oxidative decarboxylation | Sus scrofa | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | 8.5 | - |
Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | natural coenzyme | Sus scrofa | |
| NADP+ | natural coenzyme | Saccharomyces cerevisiae | |
| NADP+ | natural coenzyme | Aspergillus niger | |
| NADP+ | natural coenzyme | Mycobacterium tuberculosis | |
| NADP+ | natural coenzyme | Trypanosoma cruzi | |
| NADP+ | natural coenzyme | Citrobacter freundii | |
| NADPH | - |
Sus scrofa | |
| NADPH | - |
Saccharomyces cerevisiae | |
| NADPH | - |
Aspergillus niger | |
| NADPH | - |
Mycobacterium tuberculosis | |
| NADPH | - |
Trypanosoma cruzi | |
| NADPH | - |
Citrobacter freundii | |
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Release 2023.1 (January 2023)
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