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Literature summary for 1.1.1.418 extracted from

  • Rahier, A.; Darnet, S.; Bouvier, F.; Camara, B.; Bard, M.
    Molecular and enzymatic characterizations of novel bifunctional 3beta-hydroxysteroid dehydrogenases/C-4 decarboxylases from Arabidopsis thaliana (2006), J. Biol. Chem., 281, 27264-27277.
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain erg26 SDG200 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 NAD+ isoform 3betaHSD/D2, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.008
-
 NAD+ isoform 3betaHSD/D1, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.081
-
 3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol isoform 3betaHSD/D2, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.087
-
 4alpha-carboxy-cholest-7-en-3beta-ol isoform 3betaHSD/D2, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.112
-
 3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol isoform 3betaHSD/D1, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.134
-
 4alpha-carboxy-cholest-7-en-3beta-ol isoform 3betaHSD/D1, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.204
-
 4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol isoform 3betaHSD/D2, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.274
-
 4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol isoform 3betaHSD/D1, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.383
-
 4alpha-carboxy-cholest-7-en-3alpha-ol isoform 3betaHSD/D1, at 30°C, pH not specified in the publication Arabidopsis thaliana
0.655
-
 4alpha-carboxy-cholest-7-en-3alpha-ol isoform 3betaHSD/D2, at 30°C, pH not specified in the publication Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Arabidopsis thaliana
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 3beta-hydroxysteroid-4alpa-carboxylate + NAD+ Arabidopsis thaliana
-
 a 3-oxosteroid + CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q67ZE1
-
-
Arabidopsis thaliana Q9FX01
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha-deutero-4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
-
 Arabidopsis thaliana ? + CO2 + NADH + H+
-
 ?
4alpha-carboxy-4beta,14alpha-dimethyl-9beta,19-cycloergost-24(241)-en-3beta-ol + NAD+
-
 Arabidopsis thaliana 4alpha,14alpha-dimethyl-9beta,19-cyclo-ergost-24(241)-en-3-one + CO2 + NADH + H+
-
 ?
4alpha-carboxy-4beta-methyl-cholest-8,24-dien-3beta-ol + NAD+
-
 Arabidopsis thaliana 4alpha-methyl-cholest-8,24-dien-3-one + CO2 + NADH + H+
-
 ?
4alpha-carboxy-cholest-7-en-3alpha-ol + NAD+
-
 Arabidopsis thaliana cholest-7-en-3-one + CO2 + NADH + H+
-
 ?
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
-
 Arabidopsis thaliana cholest-7-en-3-one + CO2 + NADH + H+
-
 ?
4alpha-carboxy-stigmasta-7,24(241)-dien-3beta-ol + NAD+
-
 Arabidopsis thaliana stigmasta-7,24(241)-dien-3-one + CO2 + NADH + H+
-
 ?
a 3beta-hydroxysteroid-4alpa-carboxylate + NAD+
-
 Arabidopsis thaliana a 3-oxosteroid + CO2 + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
3beta-hydroxysteroid dehydrogenase/C-4 decarboxylase
-
 Arabidopsis thaliana
3betaHSD/D1 isoform Arabidopsis thaliana
3betaHSD/D2 isoform Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arabidopsis thaliana