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Literature summary for 1.1.1.418 extracted from

  • Rondet, S.; Taton, M.; Rahier, A.
    Identification, characterization, and partial purification of 4apha-carboxysterol-C3-dehydrogenase/C4-decarboxylase from Zea mays (1999), Arch. Biochem. Biophys., 366, 249-260 .
    View publication on PubMed
Search for more literature for 1.1.1.418

General Stability

General Stability Organism
treatment of microsomes with sodium cholate at a final concentration of 1% (10 mg/ml) leads to 58% solubilization of the enzyme activity, while Brij W-1 at a final concentration of 1% (10 mg/ml) yields 115% recovery of the initial enzyme activity Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Zea mays
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+ Zea mays
-
 a 3-oxosteroid + CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Trisacryl column chromatography, Cibacron blue 3GA agarose dye chromatography, and Sephacryl S-100 gel filtration Zea mays

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4alpha-carboxy-5alpha-stigmastane-3beta-ol + NAD+
-
 Zea mays stigmastane-3-one + CO2 + NADH + H+
-
 ?
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+
-
 Zea mays cholest-7-en-3-one + CO2 + NADH + H+
-
 ?
4alpha-[242-3H]carboxy-5alpha-stigmasta-7,24(241)-dien-3beta-ol + NAD+
-
 Zea mays ? + CO2 + NADH + H+
-
 ?
4beta,14alpha-[241-3H]dimethyl-4alpha-carboxy-5alpha-ergosta-9beta,19-cyclo-24(241)-en-3beta-ol + NAD+
-
 Zea mays ? + CO2 + NADH + H+
-
 ?
a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+
-
 Zea mays a 3-oxosteroid + CO2 + NADH + H+
-
 ?
additional information the decarboxylation reaction is independent of molecular oxygen Zea mays ?
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, enzyme in sodium cholate, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase
-
 Zea mays
4alpha-CD
-
 Zea mays

Cofactor

Cofactor Comment Organism Structure
NAD+ NAD+ is more efficient than NADP+ Zea mays