General Stability | Organism |
---|---|
treatment of microsomes with sodium cholate at a final concentration of 1% (10 mg/ml) leads to 58% solubilization of the enzyme activity, while Brij W-1 at a final concentration of 1% (10 mg/ml) yields 115% recovery of the initial enzyme activity | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Zea mays | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+ | Zea mays | - |
a 3-oxosteroid + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Trisacryl column chromatography, Cibacron blue 3GA agarose dye chromatography, and Sephacryl S-100 gel filtration | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4alpha-carboxy-5alpha-stigmastane-3beta-ol + NAD+ | - |
Zea mays | stigmastane-3-one + CO2 + NADH + H+ | - |
? | |
4alpha-carboxy-cholest-7-en-3beta-ol + NAD+ | - |
Zea mays | cholest-7-en-3-one + CO2 + NADH + H+ | - |
? | |
4alpha-[242-3H]carboxy-5alpha-stigmasta-7,24(241)-dien-3beta-ol + NAD+ | - |
Zea mays | ? + CO2 + NADH + H+ | - |
? | |
4beta,14alpha-[241-3H]dimethyl-4alpha-carboxy-5alpha-ergosta-9beta,19-cyclo-24(241)-en-3beta-ol + NAD+ | - |
Zea mays | ? + CO2 + NADH + H+ | - |
? | |
a 3beta-hydroxysteroid-4alpha-carboxylate + NAD+ | - |
Zea mays | a 3-oxosteroid + CO2 + NADH + H+ | - |
? | |
additional information | the decarboxylation reaction is independent of molecular oxygen | Zea mays | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, enzyme in sodium cholate, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase | - |
Zea mays |
4alpha-CD | - |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NAD+ is more efficient than NADP+ | Zea mays |