Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.415 extracted from

  • Li, Y.; Li, S.; Thodey, K.; Trenchard, I.; Cravens, A.; Smolke, C.
    Complete biosynthesis of noscapine and halogenated alkaloids in yeast (2018), Proc. Natl. Acad. Sci. USA, 115, E3922-E3931 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis complete biosynthesis of noscapine and halogenated alkaloids in yeast and optimizing noscapine production toward scalable manufacturing. Engineered strain contains 25 heterologous plant, bacteria, and mammalian genes and 6 mutant or overexpressed yeast genes. The noscapine biosynthetic pathway incorporates seven endomembrane-localized plant enzymes, highlighting the ability of the yeast to functionally express and properly localize large numbers of heterologous enzymes into the endoplasm reticulum. Noscapine titers were improved by 18000fold (to low mg/l levels) via a combination of enzyme engineering, pathway and strain engineering, and fermentation optimization. Microbial fermentation can be used to produce halogenated alkaloid derivatives, which can ultimately serve as potential drug leads, through feeding amino acid derivatives to strains Papaver somniferum

Cloned(Commentary)

Cloned (Comment) Organism
Saccharomyces cerevisiae CSY1060 and CSY1061 strains are used as the base strain for construction of the de novo noscapine biosynthetic strains Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
narcotine hemiacetal + NAD+ Papaver somniferum
-
noscapine + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Papaver somniferum I3PLR3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
narcotine hemiacetal + NAD+
-
Papaver somniferum noscapine + NADH + H+
-
?

Synonyms

Synonyms Comment Organism
SDR1
-
Papaver somniferum
short-chain dehydrogenase/reductase
-
Papaver somniferum