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Literature summary for 1.1.1.415 extracted from

  • Chen, X.; Facchini, P.J.
    Short-chain dehydrogenase/reductase catalyzing the final step of noscapine biosynthesis is localized to laticifers in opium poppy (2014), Plant J., 77, 173-184 .
    View publication on PubMed
Search for more literature for 1.1.1.415

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Papaver somniferum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0209
-
 narcotine hemiacetal cofactor NAD+, pH 8.0, 30°C Papaver somniferum
0.0331
-
 NAD+ pH 8.0, 30°C Papaver somniferum
0.268
-
 narcotine hemiacetal cofactor NADP+, pH 8.0, 30°C Papaver somniferum
1.249
-
 NADP+ pH 8.0, 30°C Papaver somniferum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
-
 Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
narcotine hemiacetal + NAD+ Papaver somniferum the enzyme catalyses the last step in the biosynthesis of the isoquinoline alkaloid noscapine noscapine + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Papaver somniferum I3PLR3
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Papaver somniferum

Source Tissue

Source Tissue Comment Organism Textmining
flower bud intermediate level Papaver somniferum
-
laticifer
-
 Papaver somniferum
-
leaf intermediate level Papaver somniferum
-
root least abundant in roots Papaver somniferum
-
stem NOS transcripts are detected in all opium poppy organs, but are most abundant in stems Papaver somniferum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
narcotine hemiacetal + NAD+ the enzyme catalyses the last step in the biosynthesis of the isoquinoline alkaloid noscapine Papaver somniferum noscapine + NADH + H+
-
 ?
narcotine hemiacetal + NAD+ the enzyme exhibits higher catalytic efficiency with NAD+ as the cofactor compared with NADP+ Papaver somniferum noscapine + NADH + H+
-
 r
narcotine hemiacetal + NADP+ the enzyme exhibits higher catalytic efficiency with NAD+ as the cofactor compared with NADP+ Papaver somniferum noscapine + NADPH + H+
-
 r
noscapine + NADH + H+
-
 Papaver somniferum narcotine hemiacetal + NAD+
-
 r
noscapine + NADPH + H+
-
 Papaver somniferum narcotine hemiacetal + NADP+
-
 r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.54
-
 NAD+ pH 8.0, 30°C Papaver somniferum
0.59
-
 narcotine hemiacetal pH 8.0, 30°C, cofactor NAD+ Papaver somniferum
1.69
-
 narcotine hemiacetal pH 8.0, 30°C, cofactor NADP+ Papaver somniferum
1.73
-
 NADP+ pH 8.0, 30°C Papaver somniferum

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme exhibits higher catalytic efficiency with NAD+ as the cofactor compared with NADP+ Papaver somniferum

General Information

General Information Comment Organism
malfunction suppression of NOS transcript levels in opium poppy plants subjected to virus-induced gene silencing results in a corresponding reduction in the accumulation of noscapine and an increase in narcotinehemiacetal levels in the latex Papaver somniferum
metabolism the enzyme catalyses the last step in the biosynthesis of the isoquinoline alkaloid noscapine Papaver somniferum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.38
-
 NADP+ pH 8.0, 30°C Papaver somniferum
6.296
-
 narcotine hemiacetal cofactor NADP+, pH 8.0, 30°C Papaver somniferum
16.24
-
 NAD+ pH 8.0, 30°C Papaver somniferum
28.097
-
 narcotine hemiacetal cofactor NAD+, pH 8.0, 30°C Papaver somniferum