Application | Comment | Organism |
---|---|---|
analysis | YjjN can be applied for a quantitative L-galactonate and L-gulonate detection in a coupled reaction with diaphorase | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene yjjN, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DH3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the gene results in a strain with the impaired ability to grow on L-galactonate as a sole carbon source | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
19.5 | - |
L-galactonate | pH 8.0, temperature not specified in the publication, recombinant enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | required, zinc enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactonate + NAD+ | Escherichia coli | preferred substrate | D-tagaturonate + NADH + H+ | - |
? | |
L-gulonate + NAD+ | Escherichia coli | - |
D-fructuronate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q8XB60 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DH3) by nickel affinity chromatography | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the strain O157:H7 shows the ability to grow on L-galactonate as a sole carbon source | Escherichia coli | - |
Storage Stability | Organism |
---|---|
4°C, purified recombinant His-tagged enzyme, stability of enzyme YjjN seems to be rather poor since the activity of purified protein decreases dramatically when stored at 4°C overnight | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactonate + NAD+ | preferred substrate | Escherichia coli | D-tagaturonate + NADH + H+ | - |
? | |
L-gulonate + NAD+ | - |
Escherichia coli | D-fructuronate + NADH + H+ | - |
? | |
additional information | enzyme YjjN is an L-galactonate 5-dehydrogenase having activity also for L-gulonate. Reaction product identification by GC/MS analysis. No activity with substrates of similar structure, L-fuconate and L-lyxonate. And no activity with L-mannonate, L-rhamnonate, D-gulonate, D-galacturonate, D-glucuronate, D-gluconate, D-lyxonate, D-xylonate, D-ribonate, D-arabonate, D-mannonate, D-erythronate, D-glucose, D-xylitol, D-sorbitol, glucooctanic acid, and meso-galactarate. The hydroxyl group at the C6 seems to be essential for the activity | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-galactonate dehydrogenase | - |
Escherichia coli |
YjjN | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
L-galactonate | pH 8.0, temperature not specified in the publication, recombinant enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
with L-galactonate, recombinant enzyme | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | the optimum pH for the activity with L-galactonate is pH 8.0 while the remaining activity at pH 7.0, pH 7.5, and pH 9.0 is 73%, 97%, and 0 %, respectively | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ | Escherichia coli | |
NAD+ | - |
Escherichia coli |
Organism | Comment | Expression |
---|---|---|
Escherichia coli | transcription of yjjN is upregulated during growth on L-galactonate | up |
General Information | Comment | Organism |
---|---|---|
evolution | YjjN belongs to the zinc-containing alcohol dehydrogenase family. This protein family contains dimeric or tetrameric proteins that coordinate two zinc atoms per subunit. Four conserved cysteine residues, that coordinate the zinc atoms and an aspartate residue predicting NAD+ specificity are found from the YjjN sequence (C92, C95, C98, C106, and D193) | Escherichia coli |
metabolism | the enzyme catalyzes the first step in the hexuronate catabolism in Escherichia coli, overview | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
L-galactonate | pH 8.0, temperature not specified in the publication, recombinant enzyme | Escherichia coli |