BRENDA - Enzyme Database show
show all sequences of 1.1.1.413

barS1, a gene for biosynthesis of a gamma-butyrolactone autoregulator, a microbial signaling molecule eliciting antibiotic production in Streptomyces species

Shikura, N.; Yamamura, J.; Nihira, T.; J. Bacteriol. 184, 5151-5157 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces virginiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0111
-
6-dehydro-Virginiae butanolide-A
at pH 7.5 and 25°C
Streptomyces virginiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
gel filtration
Streptomyces virginiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-dehydro-Virginiae butanolide-A + NADPH + H+
Streptomyces virginiae
-
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
Streptomyces virginiae MAFF 10-06014
-
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces virginiae
-
-
-
Streptomyces virginiae MAFF 10-06014
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-heptanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
88.9% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
3-heptanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
88.9% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae MAFF 10-06014
?
-
-
-
?
3-hexanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
102% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
3-hexanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
102% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae MAFF 10-06014
?
-
-
-
?
4-(hydroxymethyl)-3-(6-methylheptanoyl)oxolan-2-one + NADPH + H+
83.3% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
4-(hydroxymethyl)-3-(6-methylheptanoyl)oxolan-2-one + NADPH + H+
83.3% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae MAFF 10-06014
?
-
-
-
?
4-(hydroxymethyl)-3-nonanoyloxolan-2-one + NADPH + H+
75.3% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
4-(hydroxymethyl)-3-octanoyloxolan-2-one + NADPH + H+
82.8% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
4-(hydroxymethyl)-3-pentanoyloxolan-2-one + NADPH + H+
65.7% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
-
742772
Streptomyces virginiae
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
100% activity
742772
Streptomyces virginiae
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
-
742772
Streptomyces virginiae MAFF 10-06014
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
100% activity
742772
Streptomyces virginiae MAFF 10-06014
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 27000, SDS-PAGE; 2 * 27095, calculated from amino acid sequence
Streptomyces virginiae
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Streptomyces virginiae
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
50
there is 80% loss and complete loss of activity after 30 min of incubation at 35°C and at 40 to 50°C, respectively
Streptomyces virginiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptomyces virginiae
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Streptomyces virginiae
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Streptomyces virginiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Streptomyces virginiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0111
-
6-dehydro-Virginiae butanolide-A
at pH 7.5 and 25°C
Streptomyces virginiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
gel filtration
Streptomyces virginiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6-dehydro-Virginiae butanolide-A + NADPH + H+
Streptomyces virginiae
-
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
Streptomyces virginiae MAFF 10-06014
-
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-heptanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
88.9% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
3-heptanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
88.9% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae MAFF 10-06014
?
-
-
-
?
3-hexanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
102% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
3-hexanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+
102% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae MAFF 10-06014
?
-
-
-
?
4-(hydroxymethyl)-3-(6-methylheptanoyl)oxolan-2-one + NADPH + H+
83.3% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
4-(hydroxymethyl)-3-(6-methylheptanoyl)oxolan-2-one + NADPH + H+
83.3% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae MAFF 10-06014
?
-
-
-
?
4-(hydroxymethyl)-3-nonanoyloxolan-2-one + NADPH + H+
75.3% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
4-(hydroxymethyl)-3-octanoyloxolan-2-one + NADPH + H+
82.8% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
4-(hydroxymethyl)-3-pentanoyloxolan-2-one + NADPH + H+
65.7% activity compared to 6-dehydro-Virginiae butanolide-A
742772
Streptomyces virginiae
?
-
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
-
742772
Streptomyces virginiae
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
100% activity
742772
Streptomyces virginiae
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
-
742772
Streptomyces virginiae MAFF 10-06014
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
-
?
6-dehydro-Virginiae butanolide-A + NADPH + H+
100% activity
742772
Streptomyces virginiae MAFF 10-06014
(2R,3R,6S)-Virginiae butanolide-A + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 27000, SDS-PAGE; 2 * 27095, calculated from amino acid sequence
Streptomyces virginiae
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
-
Streptomyces virginiae
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
35
50
there is 80% loss and complete loss of activity after 30 min of incubation at 35°C and at 40 to 50°C, respectively
Streptomyces virginiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Streptomyces virginiae
Other publictions for EC 1.1.1.413
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741791
Lee
Identification by gene deleti ...
Streptomyces virginiae
Arch. Microbiol.
189
367-374
2008
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742772
Shikura
barS1, a gene for biosynthesi ...
Streptomyces virginiae, Streptomyces virginiae MAFF 10-06014
J. Bacteriol.
184
5151-5157
2002
-
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1
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1
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2
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13
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1
1
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1
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13
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