Any feedback?
Literature summary for 1.1.1.413 extracted from
- barS1, a gene for biosynthesis of a gamma-butyrolactone autoregulator, a microbial signaling molecule eliciting antibiotic production in Streptomyces species (2002), J. Bacteriol., 184, 5151-5157.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptomyces virginiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0111 | - |
6-dehydro-Virginiae butanolide-A | at pH 7.5 and 25°C | Streptomyces virginiae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
gel filtration | Streptomyces virginiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-dehydro-Virginiae butanolide-A + NADPH + H+ | Streptomyces virginiae | - |
(2R,3R,6S)-Virginiae butanolide-A + NADP+ | - |
? | |
| 6-dehydro-Virginiae butanolide-A + NADPH + H+ | Streptomyces virginiae MAFF 10-06014 | - |
(2R,3R,6S)-Virginiae butanolide-A + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces virginiae | - |
- |
- |
| Streptomyces virginiae MAFF 10-06014 | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-heptanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+ | 88.9% activity compared to 6-dehydro-Virginiae butanolide-A | Streptomyces virginiae | ? | - |
? | |
| 3-heptanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+ | 88.9% activity compared to 6-dehydro-Virginiae butanolide-A | Streptomyces virginiae MAFF 10-06014 | ? | - |
? | |
| 3-hexanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+ | 102% activity compared to 6-dehydro-Virginiae butanolide-A | Streptomyces virginiae | ? | - |
? | |
| 3-hexanoyl-4-(hydroxymethyl)oxolan-2-one + NADPH + H+ | 102% activity compared to 6-dehydro-Virginiae butanolide-A | Streptomyces virginiae MAFF 10-06014 | ? | - |
? | |
| 4-(hydroxymethyl)-3-(6-methylheptanoyl)oxolan-2-one + NADPH + H+ | 83.3% activity compared to 6-dehydro-Virginiae butanolide-A | Streptomyces virginiae | ? | - |
? | |
| 4-(hydroxymethyl)-3-nonanoyloxolan-2-one + NADPH + H+ | 75.3% activity compared to 6-dehydro-Virginiae butanolide-A | Streptomyces virginiae | ? | - |
? | |
| 4-(hydroxymethyl)-3-octanoyloxolan-2-one + NADPH + H+ | 82.8% activity compared to 6-dehydro-Virginiae butanolide-A | Streptomyces virginiae | ? | - |
? | |
| 4-(hydroxymethyl)-3-pentanoyloxolan-2-one + NADPH + H+ | 65.7% activity compared to 6-dehydro-Virginiae butanolide-A | Streptomyces virginiae | ? | - |
? | |
| 4-(hydroxymethyl)-3-pentanoyloxolan-2-one + NADPH + H+ | 65.7% activity compared to 6-dehydro-Virginiae butanolide-A | Streptomyces virginiae MAFF 10-06014 | ? | - |
? | |
| 6-dehydro-Virginiae butanolide-A + NADPH + H+ | - |
Streptomyces virginiae | (2R,3R,6S)-Virginiae butanolide-A + NADP+ | - |
? | |
| 6-dehydro-Virginiae butanolide-A + NADPH + H+ | 100% activity | Streptomyces virginiae | (2R,3R,6S)-Virginiae butanolide-A + NADP+ | - |
? | |
| 6-dehydro-Virginiae butanolide-A + NADPH + H+ | - |
Streptomyces virginiae MAFF 10-06014 | (2R,3R,6S)-Virginiae butanolide-A + NADP+ | - |
? | |
| 6-dehydro-Virginiae butanolide-A + NADPH + H+ | 100% activity | Streptomyces virginiae MAFF 10-06014 | (2R,3R,6S)-Virginiae butanolide-A + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 27095, calculated from amino acid sequence | Streptomyces virginiae |
| homodimer | 2 * 27000, SDS-PAGE | Streptomyces virginiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BARS1 | - |
Streptomyces virginiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Streptomyces virginiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 50 | there is 80% loss and complete loss of activity after 30 min of incubation at 35°C and at 40 to 50°C, respectively | Streptomyces virginiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptomyces virginiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Streptomyces virginiae | |
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