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Literature summary for 1.1.1.41 extracted from

  • Lv, P.; Tang, W.; Wang, P.; Cao, Z.; Zhu, G.
    Enzymatic characterization and functional implication of two structurally different isocitrate dehydrogenases from Xylella fastidiosa (2018), Biotechnol. Appl. Biochem., 65, 230-237 .
    View publication on PubMed
Search for more literature for 1.1.1.41

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Xylella fastidiosa

Protein Variants

Protein Variants Comment Organism
D268K the coenzyme specificity of the mutant is switched from NAD+ to NADP+ Xylella fastidiosa
D268K/I269Y the coenzyme specificity of the mutant is switched from NAD+ to NADP+ Xylella fastidiosa
D268K/I269Y/A275V the coenzyme specificity of the mutant is switched from NAD+ to NADP+ Xylella fastidiosa

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 7.9% residual activity at 2 mM Xylella fastidiosa
Co2+ 5.2% residual activity at 2 mM Xylella fastidiosa
Cu2+ complete inhibition at 2 mM Xylella fastidiosa
K+ complete inhibition at 2 mM Xylella fastidiosa
Na+ complete inhibition at 2 mM Xylella fastidiosa
Ni2+ complete inhibition at 2 mM Xylella fastidiosa
Zn2+ complete inhibition at 2 mM Xylella fastidiosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
 NADP+ mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C Xylella fastidiosa
0.087
-
 NADP+ mutant enzyme D268K/I269Y, at pH 8.0 and 25°C Xylella fastidiosa
0.101
-
 NADP+ mutant enzyme D268K, at pH 8.0 and 25°C Xylella fastidiosa
0.121
-
 NAD+ wild type enzyme, at pH 8.0 and 25°C Xylella fastidiosa
0.384
-
 NAD+ mutant enzyme D268K, at pH 8.0 and 25°C Xylella fastidiosa
2.339
-
 NADP+ wild type enzyme, at pH 8.0 and 25°C Xylella fastidiosa
3.15
-
 NAD+ mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C Xylella fastidiosa
4.413
-
 NAD+ mutant enzyme D268K/I269Y, at pH 8.0 and 25°C Xylella fastidiosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ is the most favored divalent cation. Mg2+ can be largely replaced by Mn2+ (69.2% activity). 2 mM is used in assay conditions Xylella fastidiosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75500
-
 MALDI-TOF mass spectrometry Xylella fastidiosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NAD+ Xylella fastidiosa
-
 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Xylella fastidiosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
metal affinity resin column chromatography Xylella fastidiosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3
-
 with NADP+ as cosubstrate, at pH 8.0 and 25°C Xylella fastidiosa
96.8
-
 with NAD+ as cosubstrate, at pH 8.0 and 25°C Xylella fastidiosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NAD+
-
 Xylella fastidiosa 2-oxoglutarate + CO2 + NADH + H+
-
 ?
isocitrate + NADP+
-
 Xylella fastidiosa 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 38000, SDS-PAGE Xylella fastidiosa

Synonyms

Synonyms Comment Organism
NAD+-dependent IDH
-
 Xylella fastidiosa
NADH-IDH
-
 Xylella fastidiosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Xylella fastidiosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 47 the enzyme is stable below 40°C and half of its activity is lost after incubation at 47°C for 20 min Xylella fastidiosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1
-
 NADP+ wild type enzyme, at pH 8.0 and 25°C Xylella fastidiosa
6.9
-
 NADP+ mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C Xylella fastidiosa
11.8
-
 NAD+ mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C Xylella fastidiosa
30.4
-
 NADP+ mutant enzyme D268K/I269Y, at pH 8.0 and 25°C Xylella fastidiosa
37.3
-
 NAD+ mutant enzyme D268K/I269Y, at pH 8.0 and 25°C Xylella fastidiosa
38.5
-
 NADP+ mutant enzyme D268K, at pH 8.0 and 25°C Xylella fastidiosa
52.8
-
 NAD+ mutant enzyme D268K, at pH 8.0 and 25°C Xylella fastidiosa
74.6
-
 NAD+ wild type enzyme, at pH 8.0 and 25°C Xylella fastidiosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 the enzyme retains more than 95% activity at pH 8.0-9.0 with Mg2+ Xylella fastidiosa

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme displays 206fold preferences for NAD+ over NADP+ Xylella fastidiosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3
-
 NADP+ wild type enzyme, at pH 8.0 and 25°C Xylella fastidiosa
4
-
 NAD+ mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C Xylella fastidiosa
8
-
 NAD+ mutant enzyme D268K/I269Y, at pH 8.0 and 25°C Xylella fastidiosa
138
-
 NAD+ mutant enzyme D268K, at pH 8.0 and 25°C Xylella fastidiosa
223
-
 NADP+ mutant enzymeD268K/I269Y/A275V, at pH 8.0 and 25°C Xylella fastidiosa
349
-
 NADP+ mutant enzyme D268K/I269Y, at pH 8.0 and 25°C Xylella fastidiosa
381
-
 NADP+ mutant enzyme D268K, at pH 8.0 and 25°C Xylella fastidiosa
617
-
 NAD+ wild type enzyme, at pH 8.0 and 25°C Xylella fastidiosa