Activating Compound | Comment | Organism | Structure |
---|---|---|---|
citrate | 110% activity at 2 mM | Campylobacter concisus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Campylobacter concisus |
Protein Variants | Comment | Organism |
---|---|---|
L580H/L591R/A640R | the mutant has a 21fold lower Km value for NADP+ when compared to the original enzyme. The turnover rate (kcat) towards isocitrate of the mutant is reduced by more than 50% as compared to the wild type enzyme and even though the mutant is capable of using NADP+ for catalysis, ist catalytic efficiency (kcat/Km) for isocitrate is relatively low, being half of that for the wild type enzyme as using NAD+ | Campylobacter concisus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | complete inhibition at 10 mM | Campylobacter concisus | |
ADP | 85% residual activity at 2 mM | Campylobacter concisus | |
AMP | 79% residual activity at 2 mM | Campylobacter concisus | |
ATP | 68% residual activity at 2 mM | Campylobacter concisus | |
Ca2+ | 1.9% residual activity at 2 mM | Campylobacter concisus | |
cis-aconitate | 91% residual activity at 2 mM | Campylobacter concisus | |
Co2+ | 8.7% residual activity at 2 mM | Campylobacter concisus | |
Cu2+ | 1.3% residual activity at 2 mM | Campylobacter concisus | |
fumarate | 96% residual activity at 2 mM | Campylobacter concisus | |
K+ | 1.7% residual activity at 2 mM | Campylobacter concisus | |
L-glutamate | 90% residual activity at 2 mM | Campylobacter concisus | |
L-glutamine | 96% residual activity at 2 mM | Campylobacter concisus | |
additional information | not influenced by pyruvate and malate | Campylobacter concisus | |
Na+ | 3.2% residual activity at 2 mM | Campylobacter concisus | |
Ni2+ | 4.4% residual activity at 2 mM | Campylobacter concisus | |
oxaloacetate | 98% residual activity at 2 mM | Campylobacter concisus | |
succinate | 95% residual activity at 2 mM | Campylobacter concisus | |
Zn2+ | 0.4% residual activity at 2 mM | Campylobacter concisus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.031 | - |
isocitrate | mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C | Campylobacter concisus | |
0.038 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Campylobacter concisus | |
0.088 | - |
NAD+ | wild type enzyme, at pH 7.5 and 25°C | Campylobacter concisus | |
0.207 | - |
NADP+ | mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C | Campylobacter concisus | |
4.3 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Campylobacter concisus | |
4.5 | - |
NAD+ | mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C | Campylobacter concisus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ can take the place of Mn2+ with 44.8% eficiency. Mg2+ promotes 94% activity in the presence of Mn2+. 2 mM used in assay conditions | Campylobacter concisus | |
Mn2+ | 100% activity, 2 mM used in assay conditions | Campylobacter concisus | |
additional information | in the presence of either Mn2+ or Mg2+, neither K+ nor Na+ affects enzyme activity | Campylobacter concisus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Campylobacter concisus | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | Campylobacter concisus DSM9716 | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter concisus | - |
- |
- |
Campylobacter concisus DSM9716 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Campylobacter concisus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.3 | - |
wild type enzyme, with NADP+ as cosubstrate, at pH 7.5 and 25°C | Campylobacter concisus |
49.3 | - |
wild type enzyme, with NAD+ as cosubstrate, at pH 7.5 and 25°C | Campylobacter concisus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | - |
Campylobacter concisus | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NAD+ | - |
Campylobacter concisus DSM9716 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
isocitrate + NADP+ | the enzyme has weak activity with NADP+ | Campylobacter concisus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? | |
isocitrate + NADP+ | the enzyme has weak activity with NADP+ | Campylobacter concisus DSM9716 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 80000, His6-tagged recombinant enzyme, SDS-PAGE | Campylobacter concisus |
monomer | 1 * 81000, calculated from amino acid sequence | Campylobacter concisus |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Campylobacter concisus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Campylobacter concisus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | more than 60% activity between 45 and 60°C | Campylobacter concisus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 47 | the enzyme is stable below 45°C. The activity of the enzyme drops quickly above 45°C. Incubation at 47°C for 20 min causes 54% and 51% loss of activity with Mn2+ or Mg2+, respectively | Campylobacter concisus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Campylobacter concisus | |
37 | - |
isocitrate | mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C | Campylobacter concisus | |
43 | - |
NADP+ | mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C | Campylobacter concisus | |
78 | - |
NAD+ | wild type enzyme, at pH 7.5 and 25°C | Campylobacter concisus | |
79 | - |
NAD+ | mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C | Campylobacter concisus | |
85 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Campylobacter concisus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Campylobacter concisus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | more than 60% activity between pH 6.5 and 8.0 | Campylobacter concisus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the recombinant enzyme has a significant high coenzyme preference for NAD+ | Campylobacter concisus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.7 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Campylobacter concisus | |
17.7 | - |
NAD+ | mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C | Campylobacter concisus | |
208 | - |
NADP+ | mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C | Campylobacter concisus | |
886 | - |
NAD+ | wild type enzyme, at pH 7.5 and 25°C | Campylobacter concisus | |
1200 | - |
isocitrate | mutant enzyme L580H/L591R/A640R, at pH 7.5 and 25°C | Campylobacter concisus | |
2200 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Campylobacter concisus |