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Literature summary for 1.1.1.41 extracted from

  • Hatzopoulos, G.N.; Kefala, G.; Mueller-Dieckmann, J.
    Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of isocitrate dehydrogenase 2 (Rv0066c) from Mycobacterium tuberculosis (2008), Acta Crystallogr. Sect. F, 64, 1139-1142.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)RP cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method, using 3-5% (w/v) PEG 20000, 0.1 M MES pH 5.7 and 0.2 M CaCl2 Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83181
-
3 * 83181, calculated from amino acid sequence Mycobacterium tuberculosis
250000
-
Superose 6 gel filtration Mycobacterium tuberculosis
260000
-
dynamic light scattering Mycobacterium tuberculosis
290000
-
Superdex 200 gel filtration Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Trap chelating column chromatography and Superdex 200 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-isocitrate + NAD+
-
Mycobacterium tuberculosis 2-oxoglutarate + CO2 + NADH
-
?
D-isocitrate + NAD+
-
Mycobacterium tuberculosis H37Rv 2-oxoglutarate + CO2 + NADH
-
?

Subunits

Subunits Comment Organism
trimer 3 * 83181, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
Icd-2
-
Mycobacterium tuberculosis
isocitrate dehydrogenase 2
-
Mycobacterium tuberculosis
Rv0066c
-
Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Mycobacterium tuberculosis