Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by NADP+, HCO3-, and 2-oxoglutarate | Pisum sativum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not affected by NADP+, HCO3-, and 2-oxoglutarate | Pisum sativum | |
NADH | competitive | Pisum sativum | |
NADPH | noncompetitive | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
isocitrate | pH 7.5 | Pisum sativum | |
0.2 | - |
NAD+ | pH 7.5 | Pisum sativum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Pisum sativum | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | Pisum sativum | pyridine nucleotide contents in mitochondria and cytosol, regulation, overview | 2-oxoglutarate + CO2 + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
cv. 294 040 Märgärt Kelvedon Wonder | - |
Purification (Comment) | Organism |
---|---|
partially by mitochondrion isolation in a Percoll gradient, and ammonium sulfate precipitation in the 60-80% fraction, DEAE cellulose chromatography, and desalting | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | green and etiolated | Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.084 | - |
mitochondria from green leaves, forward reaction | Pisum sativum |
0.096 | - |
mitochondria from etiolated leaves, forward reaction | Pisum sativum |
Storage Stability | Organism |
---|---|
-80°C, partially purified enzyme, several months, stable | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NAD+ | pyridine nucleotide contents in mitochondria and cytosol, regulation, overview | Pisum sativum | 2-oxoglutarate + CO2 + NADH + H+ | - |
ir | |
threo-Ds-isocitrate + NAD+ | - |
Pisum sativum | 2-oxoglutarate + CO2 + NADH | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent ICDH | - |
Pisum sativum |
NAD-dependent isocitrate dehydrogenase | - |
Pisum sativum |
NAD-ICDH | - |
Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Pisum sativum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for | Pisum sativum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
NADH | pH 7.5 | Pisum sativum | |
0.26 | - |
NADPH | pH 7.5 | Pisum sativum |