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Literature summary for 1.1.1.41 extracted from
- Regulation of NAD(+)-dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowia lipolytica (2004), Biochemistry, 69, 1391-1398.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AMP | - |
Yarrowia lipolytica |  |
General Stability
| General Stability | Organism |
|---|---|
| 20% glycerol stabilizes during storage | Yarrowia lipolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glyoxylate | slight inhibition | Yarrowia lipolytica | |
| NADH | complete inhibition at 0.2 mM, competitive | Yarrowia lipolytica | |
| oxaloacetate | slight inhibition | Yarrowia lipolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.136 | - |
NAD+ | pH 7.4, 25°C | Yarrowia lipolytica | |
| 0.581 | - |
isocitrate | pH 7.4, 25°C | Yarrowia lipolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Yarrowia lipolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
8 * 52000, SDS-PAGE | Yarrowia lipolytica |
| 412000 | - |
gel filtration | Yarrowia lipolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NAD+ | Yarrowia lipolytica | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
| additional information | Yarrowia lipolytica | enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yarrowia lipolytica | - |
citrate-overproducing yeast, strain BKM Y-2373 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 129fold to homogeneity by ammonium sulfate fractionation, hydrophobic chromatography on an octyl resin, and gel filtration | Yarrowia lipolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.15 | - |
enzyme extract of cells in exponential growth phase | Yarrowia lipolytica |
| 0.16 | - |
enzyme extract of cells in stationary growth phase | Yarrowia lipolytica |
| 0.17 | - |
enzyme extract of cells in growth phase with acid synthesis | Yarrowia lipolytica |
| 22 | - |
purified enzyme | Yarrowia lipolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isocitrate + NAD+ | - |
Yarrowia lipolytica | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
| additional information | enzyme is involved in tricarboxylic acid cycle and plays a key role in the regulation of biosynthesis of citrate and isocitrate, yeast is overproducing citrate under nitrogen deficiency in presence of glucose | Yarrowia lipolytica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 52000, SDS-PAGE | Yarrowia lipolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NAD+-dependent isocitrate dehydrogenase | - |
Yarrowia lipolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Yarrowia lipolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Yarrowia lipolytica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Yarrowia lipolytica | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
NADH | pH 7.4, 25°C | Yarrowia lipolytica | |
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