Protein Variants | Comment | Organism |
---|---|---|
H142A | site-directed mutagenesis, a dimeric tetramer interface mutant | Homo sapiens |
H142A/D568A | site-directed mutagenesis, a dimeric tetramer interface mutant. The mutant dimer completely dissociates into monomers after a 2.5 M urea treatment | Homo sapiens |
H51A/D139A | site-directed mutagenesis, a dimeric dimer interface mutant | Homo sapiens |
H51A/D90A | site-directed mutagenesis, a dimeric dimer interface mutant. The mutant dimer completely dissociates into monomers after a 1.5 M urea treatment | Homo sapiens |
W572A | site-directed mutagenesis, a dimeric tetramer interface mutant | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Mg2+ does not influence the thermal stability of the enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is first dissociated from a tetramer to dimers before the 2 M urea treatment, and the dimers then dissociated into monomers before the 2.5 M urea treatment | Homo sapiens |
tetramer | c-NADP-ME has a dimer-dimer quaternary structure in which the dimer interface associates more tightly than the tetramer interface | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
c-NADP-ME | - |
Homo sapiens |
cytosolic malic enzyme | - |
Homo sapiens |
cytosolic NADP+-dependent malic enzyme | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Mg2+ does not influence the thermal stability of the enzyme | Homo sapiens |
52 | 64 | thermal denaturation of c-NADP-ME wild-type and the interface mutants, overview | Homo sapiens |