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Literature summary for 1.1.1.40 extracted from
- Purification and properties of malic enzyme from herring Clupea harengus spermatozoa (2013), Comp. Biochem. Physiol. B, 164, 216-220.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates | Cyprinus carpio |  |
| Mn2+ | activates | Salmo salar | |
| Mn2+ | activates | Clarias gariepinus | |
| Mn2+ | activates | Clupea harengus | |
| Mn2+ | activates | Salmo trutta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
4 * 64000, SDS-PAGE | Clupea harengus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Cyprinus carpio | - |
pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | Salmo salar | - |
pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | Clarias gariepinus | - |
pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | Clupea harengus | - |
pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | Clupea harengus | - |
pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | Salmo trutta | - |
pyruvate + CO2 + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clarias gariepinus | - |
- |
- |
| Clupea harengus | - |
- |
- |
| Clupea harengus | - |
from the Baltic Sea | - |
| Cyprinus carpio | - |
- |
- |
| Salmo salar | - |
- |
- |
| Salmo trutta | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme by anion exchange and affinity chromatography | Clupea harengus |
| native NADP-specific malic enzyme by anion exchange and 2'5'-ADP affinity chromatography | Clupea harengus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spermatozoon | two molecular forms of malic enzyme are present in carp spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Cyprinus carpio | - |
| spermatozoon | two molecular forms of malic enzyme are present in catfish spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Clarias gariepinus | - |
| spermatozoon | two molecular forms of malic enzyme are present in herring spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Clupea harengus | - |
| spermatozoon | two molecular forms of malic enzyme are present in salmon spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Salmo salar | - |
| spermatozoon | two molecular forms of malic enzyme are present in salmon spermatozoa: an NAD-preferring malic enzyme with very high activity and an NADP-specific malic enzyme (EC 1.1.1.40) with much lower activity (ratio about 33:1) | Salmo trutta | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.008 | - |
purified native enzyme, pH 7.5, 25°C | Clarias gariepinus |
| 0.023 | - |
purified native enzyme, pH 7.5, 25°C | Salmo trutta |
| 0.026 | - |
purified native enzyme, pH 7.5, 25°C | Salmo salar |
| 0.045 | - |
purified native enzyme, pH 7.5, 25°C | Cyprinus carpio |
| 0.213 | - |
purified native enzyme, pH 7.5, 25°C | Clupea harengus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Cyprinus carpio | pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | - |
Salmo salar | pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | - |
Clarias gariepinus | pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | - |
Clupea harengus | pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | - |
Clupea harengus | pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | - |
Salmo trutta | pyruvate + CO2 + NADPH | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, SDS-PAGE | Clupea harengus |
| tetramer | 4 * 64000, SDS-PAGE | Clupea harengus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADP-specific ME | - |
Clupea harengus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Cyprinus carpio |
| 25 | - |
assay at | Salmo salar |
| 25 | - |
assay at | Clarias gariepinus |
| 25 | - |
assay at | Clupea harengus |
| 25 | - |
assay at | Salmo trutta |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
malate decarboxylation | Clupea harengus |
| 7.5 | - |
assay at | Cyprinus carpio |
| 7.5 | - |
assay at | Salmo salar |
| 7.5 | - |
assay at | Clarias gariepinus |
| 7.5 | - |
assay at | Clupea harengus |
| 7.5 | - |
assay at | Salmo trutta |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | activity range, profile overview | Clupea harengus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Cyprinus carpio | |
| NADP+ | - |
Salmo salar | |
| NADP+ | - |
Clarias gariepinus | |
| NADP+ | - |
Clupea harengus | |
| NADP+ | - |
Salmo trutta | |
| NADP+ | dependent on | Clupea harengus | |
| NADPH | - |
Cyprinus carpio | |
| NADPH | - |
Salmo salar | |
| NADPH | - |
Clarias gariepinus | |
| NADPH | - |
Clupea harengus | |
| NADPH | - |
Salmo trutta | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Cyprinus carpio |
| metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Salmo salar |
| metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Clarias gariepinus |
| metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Clupea harengus |
| metabolism | fish spermatozoa contain a glycolytic pathway, tricarboxylic acid cycle and oxidative phosphorylation system, all of which are key pathways contributing to ATP synthesis, involving the enzyme | Salmo trutta |
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