Cloned (Comment) | Organism |
---|---|
gene Sco5261, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expressionin Escherichia coli strain BL21(DE3) | Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.107 | - |
NADP+ | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor | |
6.4 | - |
(S)-malate | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor | |
18.1 | - |
pyruvate | pH 8.0, 30°C, recombinant enzyme | Streptomyces coelicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activates | Streptomyces coelicolor |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Streptomyces coelicolor | - |
pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | Streptomyces coelicolor M145 | - |
pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces coelicolor | Q9F3K4 | gene Sco5261 | - |
Streptomyces coelicolor M145 | Q9F3K4 | gene Sco5261 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Streptomyces coelicolor | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | - |
Streptomyces coelicolor M145 | pyruvate + CO2 + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
malic enzyme-NADP | - |
Streptomyces coelicolor |
ME-NADP | - |
Streptomyces coelicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces coelicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces coelicolor |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | dependent on | Streptomyces coelicolor | |
NADPH | - |
Streptomyces coelicolor |
General Information | Comment | Organism |
---|---|---|
malfunction | the double Sco2951 Sco5261 mutant, deficient in ME-NAD, EC 1.1.1.39, and ME-NADP activity, display a strong reduction in the production of the polyketide antibiotic actinorhodin. Additionally, the Sco2951/Sco5261 mutant shows a decrease in stored triacylglcerides during exponential growth | Streptomyces coelicolor |
physiological function | the enzyme plays a role in antibiotic and triacylglycerol production, e.g. production of the polyketide antibiotic actinorhodin, overview | Streptomyces coelicolor |