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Literature summary for 1.1.1.40 extracted from

  • Hsieh, J.Y.; Chen, S.H.; Hung, H.C.
    Functional roles of the tetramer organization of malic enzyme (2009), J. Biol. Chem., 284, 18096-18105.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D139A dimeric mutant enzyme with reduced activity compared to the wild type enzyme Homo sapiens
D568A dimeric or tetrameric mutant enzyme with increased activity compared to the wild type enzyme Homo sapiens
D90A dimeric or tetrameric mutant enzyme with reduced activity compared to the wild type enzyme Homo sapiens
H142A dimeric mutant enzyme with increased activity compared to the wild type enzyme Homo sapiens
H142A/D568A dimeric mutant enzyme with reduced activity compared to the wild type enzyme Homo sapiens
H51A dimeric or tetrameric mutant enzyme with wild type activity Homo sapiens
H51A/D139A dimeric or tetrameric mutant enzyme with reduced activity compared to the wild type enzyme Homo sapiens
H51A/D90A dimeric or tetrameric mutant enzyme with reduced activity compared to the wild type enzyme Homo sapiens
W572A dimeric mutant enzyme with slightly reduced activity compared to the wild type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0037
-
NADP+ mutant enzyme H51A/D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.0039
-
NADP+ mutant enzyme D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.004
-
NADP+ mutant enzyme H142A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.004
-
NADP+ mutant enzyme H51A/D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.0042
-
NADP+ mutant enzyme H142A/D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.0048
-
NADP+ mutant enzyme D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.005
-
NADP+ mutant enzyme D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.005
-
NADP+ mutant enzyme W572A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.0072
-
NADP+ mutant enzyme H51A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.009
-
NADP+ wild type enzyme, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.49
-
(S)-malate wild type enzyme, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.68
-
(S)-malate mutant enzyme D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.84
-
(S)-malate mutant enzyme H51A/D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.89
-
(S)-malate mutant enzyme H142A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.92
-
(S)-malate mutant enzyme H142A/D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.95
-
(S)-malate mutant enzyme D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.97
-
(S)-malate mutant enzyme D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
0.98
-
(S)-malate mutant enzyme W572A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
1.03
-
(S)-malate mutant enzyme H51A/D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
1.1
-
(S)-malate mutant enzyme H51A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Homo sapiens pyruvate + CO2 + NADPH
-
?

Subunits

Subunits Comment Organism
dimer the dimeric form of c-NADP-ME is as active as tetramers, analytical ultracentrifugation Homo sapiens
tetramer c-NADP-ME exists mainly as a tetramer, analytical ultracentrifugation Homo sapiens

Synonyms

Synonyms Comment Organism
c-NADP-ME
-
Homo sapiens
cytosolic NADP+-dependent malic enzyme
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
84.83
-
(S)-malate mutant enzyme H51A/D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
94.57
-
(S)-malate mutant enzyme D90A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
95.53
-
(S)-malate mutant enzyme D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
98.03
-
(S)-malate mutant enzyme H142A/D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
105.3
-
(S)-malate mutant enzyme H51A/D139A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
106
-
(S)-malate mutant enzyme W572A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
109.5
-
(S)-malate mutant enzyme H51A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
110.9
-
(S)-malate wild type enzyme, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
122.7
-
(S)-malate mutant enzyme H142A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens
136.9
-
(S)-malate mutant enzyme D568A, in 50 mM Tris-HCl, pH 7.4, at 30°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens