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Literature summary for 1.1.1.40 extracted from

  • Gerrard Wheeler, M.C.; Arias, C.L.; Tronconi, M.A.; Maurino, V.G.; Andreo, C.S.; Drincovich, M.F.
    Arabidopsis thaliana NADP-malic enzyme isoforms: high degree of identity but clearly distinct properties (2008), Plant Mol. Biol., 67, 231-242.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
aspartate isozyme NADP-ME2 activity is highly stimulated at both low and high concentrations of 0.5 mM and 2 mM, respectively Arabidopsis thaliana
CoA activates isozyme NADP-ME2 Arabidopsis thaliana
fumarate isozyme NADP-ME2 activity is highly stimulated at both low and high concentrations of 0.5 mM and 2 mM, respectively, twofold activation of at 0.5 mM Arabidopsis thaliana
succinate isozyme NADP-ME2 activity is highly stimulated at both low and high concentrations of 0.5 mM and 2 mM, respectively, partial activation of isozyme NADP-ME1 at 2 mM Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant isozymes NADP-ME2 in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of deletion mutants of isozyme NADP-ME2 Arabidopsis thaliana
R115A site-directed mutagenesis, mutation of isozyme NADP-ME2, the mutant shows similar kinetics as the wild-type isozyme NADP-ME2, but loses the activation ability of fumarate Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA inhibits isozyme NADP-ME1 Arabidopsis thaliana
fumarate inhibits isozymes NADP-ME1 , NADP-ME3 and NADP-ME4 Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of isozymes at different pH, overview Arabidopsis thaliana
0.0065
-
NADP+ pH 7.5, 30°C, isozyme NADP-ME3 Arabidopsis thaliana
0.0102
-
NADP+ pH 7.5, 30°C, isozyme NADP-ME4 Arabidopsis thaliana
0.0721
-
NADP+ pH 7.5, 30°C, isozyme NADP-ME2 Arabidopsis thaliana
0.205
-
NADP+ pH 7.5, 30°C, isozyme NADP-ME1 Arabidopsis thaliana
0.23
-
(S)-malate pH 7.5, 30°C, isozyme NADP-ME4 Arabidopsis thaliana
0.83
-
(S)-malate pH 7.5, 30°C, isozyme NADP-ME3 Arabidopsis thaliana
2.96
-
(S)-malate pH 7.5, 30°C, isozyme NADP-ME1 Arabidopsis thaliana
3.33
-
(S)-malate pH 7.5, 30°C, isozyme NADP-ME2 Arabidopsis thaliana
16.9
-
pyruvate pH 7.0, 30°C, isozyme NADP-ME1 Arabidopsis thaliana
26.3
-
pyruvate pH 7.0, 30°C, isozyme NADP-ME4 Arabidopsis thaliana
48.2
-
pyruvate pH 7.0, 30°C, isozyme NADP-ME3 Arabidopsis thaliana
138.9
-
pyruvate pH 7.0, 30°C, isozyme NADP-ME2 Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast isozyme NADP-ME4 Arabidopsis thaliana 9507
-
cytosol isozymes NADP-ME1-3 Arabidopsis thaliana 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Arabidopsis thaliana
-
pyruvate + CO2 + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
4 isozymes NADP-ME1-4
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-
additional information NADP-ME2 and 4 are constitutively expressed, while NADP-ME1 is restricted to secondary roots and NADP-ME3 to trichomes and pollen Arabidopsis thaliana
-
pollen isozyme NADP-ME3 Arabidopsis thaliana
-
root isozyme NADP-ME1 Arabidopsis thaliana
-
trichome isozyme NADP-ME3 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Arabidopsis thaliana pyruvate + CO2 + NADPH
-
r
(S)-malate + NADP+ NADP-ME2 is the isozyme with the highest catalytic efficiency for the reverse reaction, while NADP-ME4 presents higher kcat for the reverse reaction than for the forward reaction Arabidopsis thaliana pyruvate + CO2 + NADPH
-
r

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
NADP-malic enzyme
-
Arabidopsis thaliana
NADP-ME
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.5
-
pyruvate pH 7.0, 30°C, isozyme NADP-ME1 Arabidopsis thaliana
38.7
-
(S)-malate pH 7.5, 30°C, isozyme NADP-ME1 Arabidopsis thaliana
75
-
pyruvate pH 7.0, 30°C, isozyme NADP-ME2 Arabidopsis thaliana
151.3
-
(S)-malate pH 7.5, 30°C, isozyme NADP-ME4 Arabidopsis thaliana
237
-
pyruvate pH 7.0, 30°C, isozyme NADP-ME3 Arabidopsis thaliana
268.1
-
(S)-malate pH 7.5, 30°C, isozyme NADP-ME3 Arabidopsis thaliana
284.1
-
pyruvate pH 7.0, 30°C, isozyme NADP-ME4 Arabidopsis thaliana
324.1
-
(S)-malate pH 7.5, 30°C, isozyme NADP-ME2 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at, reverse reaction Arabidopsis thaliana
7.5
-
assay at, forward reaction Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
5 8.9
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana