Activating Compound | Comment | Organism | Structure |
---|---|---|---|
aspartate | isozyme NADP-ME2 activity is highly stimulated at both low and high concentrations of 0.5 mM and 2 mM, respectively | Arabidopsis thaliana | |
CoA | activates isozyme NADP-ME2 | Arabidopsis thaliana | |
fumarate | isozyme NADP-ME2 activity is highly stimulated at both low and high concentrations of 0.5 mM and 2 mM, respectively, twofold activation of at 0.5 mM | Arabidopsis thaliana | |
succinate | isozyme NADP-ME2 activity is highly stimulated at both low and high concentrations of 0.5 mM and 2 mM, respectively, partial activation of isozyme NADP-ME1 at 2 mM | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant isozymes NADP-ME2 in Escherichia coli | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of deletion mutants of isozyme NADP-ME2 | Arabidopsis thaliana |
R115A | site-directed mutagenesis, mutation of isozyme NADP-ME2, the mutant shows similar kinetics as the wild-type isozyme NADP-ME2, but loses the activation ability of fumarate | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | inhibits isozyme NADP-ME1 | Arabidopsis thaliana | |
fumarate | inhibits isozymes NADP-ME1 , NADP-ME3 and NADP-ME4 | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of isozymes at different pH, overview | Arabidopsis thaliana | |
0.0065 | - |
NADP+ | pH 7.5, 30°C, isozyme NADP-ME3 | Arabidopsis thaliana | |
0.0102 | - |
NADP+ | pH 7.5, 30°C, isozyme NADP-ME4 | Arabidopsis thaliana | |
0.0721 | - |
NADP+ | pH 7.5, 30°C, isozyme NADP-ME2 | Arabidopsis thaliana | |
0.205 | - |
NADP+ | pH 7.5, 30°C, isozyme NADP-ME1 | Arabidopsis thaliana | |
0.23 | - |
(S)-malate | pH 7.5, 30°C, isozyme NADP-ME4 | Arabidopsis thaliana | |
0.83 | - |
(S)-malate | pH 7.5, 30°C, isozyme NADP-ME3 | Arabidopsis thaliana | |
2.96 | - |
(S)-malate | pH 7.5, 30°C, isozyme NADP-ME1 | Arabidopsis thaliana | |
3.33 | - |
(S)-malate | pH 7.5, 30°C, isozyme NADP-ME2 | Arabidopsis thaliana | |
16.9 | - |
pyruvate | pH 7.0, 30°C, isozyme NADP-ME1 | Arabidopsis thaliana | |
26.3 | - |
pyruvate | pH 7.0, 30°C, isozyme NADP-ME4 | Arabidopsis thaliana | |
48.2 | - |
pyruvate | pH 7.0, 30°C, isozyme NADP-ME3 | Arabidopsis thaliana | |
138.9 | - |
pyruvate | pH 7.0, 30°C, isozyme NADP-ME2 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | isozyme NADP-ME4 | Arabidopsis thaliana | 9507 | - |
cytosol | isozymes NADP-ME1-3 | Arabidopsis thaliana | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Arabidopsis thaliana | - |
pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
4 isozymes NADP-ME1-4 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
additional information | NADP-ME2 and 4 are constitutively expressed, while NADP-ME1 is restricted to secondary roots and NADP-ME3 to trichomes and pollen | Arabidopsis thaliana | - |
pollen | isozyme NADP-ME3 | Arabidopsis thaliana | - |
root | isozyme NADP-ME1 | Arabidopsis thaliana | - |
trichome | isozyme NADP-ME3 | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Arabidopsis thaliana | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | NADP-ME2 is the isozyme with the highest catalytic efficiency for the reverse reaction, while NADP-ME4 presents higher kcat for the reverse reaction than for the forward reaction | Arabidopsis thaliana | pyruvate + CO2 + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
NADP-malic enzyme | - |
Arabidopsis thaliana |
NADP-ME | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.5 | - |
pyruvate | pH 7.0, 30°C, isozyme NADP-ME1 | Arabidopsis thaliana | |
38.7 | - |
(S)-malate | pH 7.5, 30°C, isozyme NADP-ME1 | Arabidopsis thaliana | |
75 | - |
pyruvate | pH 7.0, 30°C, isozyme NADP-ME2 | Arabidopsis thaliana | |
151.3 | - |
(S)-malate | pH 7.5, 30°C, isozyme NADP-ME4 | Arabidopsis thaliana | |
237 | - |
pyruvate | pH 7.0, 30°C, isozyme NADP-ME3 | Arabidopsis thaliana | |
268.1 | - |
(S)-malate | pH 7.5, 30°C, isozyme NADP-ME3 | Arabidopsis thaliana | |
284.1 | - |
pyruvate | pH 7.0, 30°C, isozyme NADP-ME4 | Arabidopsis thaliana | |
324.1 | - |
(S)-malate | pH 7.5, 30°C, isozyme NADP-ME2 | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, reverse reaction | Arabidopsis thaliana |
7.5 | - |
assay at, forward reaction | Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.9 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |