Cloned (Comment) | Organism |
---|---|
expression of His-tagged chimeric NADP-ME mutants in Escherichia coli strain BL21(DE3) | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of chimerical NADP-ME sequences obtained from C4 and non-C4 isozymes | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | competitive | Zea mays | |
citrate | competitive | Zea mays | |
malate | inhibition at high concentrations at pH 7.0, but not at pH 8.0 | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant chimeric mutant enzymes, overview | Zea mays | |
0.04 | - |
NADP+ | pH 7.0, wild-type C4-NADP-ME isozyme | Zea mays | |
0.04 | - |
(S)-malate | pH 7.0, wild-type C4-NADP-ME isozyme | Zea mays | |
0.51 | - |
NADP+ | pH 7.0, wild-type non-C4-NADP-ME isozyme | Zea mays | |
0.51 | - |
(S)-malate | pH 7.0, wild-type non-C4-NADP-ME isozyme | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Zea mays | 9507 | - |
cytosol | - |
Zea mays | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Zea mays | - |
pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
C4 NADP-ME and non-C4 NADP-ME isozymes, i.e. L and R isozymes | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged chimeric NADP-ME mutants from Escherichia coli strain BL21(DE3) by two different steps of affinity chromatography to homogeneity | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Zea mays | pyruvate + CO2 + NADPH | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000-66000, recombinant chimeric enzymes, SDS-PAGE | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
C4 photosynthetic NADP-malic enzyme | - |
Zea mays |
C4-NADP-malic enzyme | - |
Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
104.4 | - |
(S)-malate | pH 7.0, wild-type C4-NADP-ME isozyme | Zea mays | |
151.7 | - |
(S)-malate | pH 7.0, wild-type non-C4-NADP-ME isozyme | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Zea mays | |
NADPH | - |
Zea mays |