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Literature summary for 1.1.1.40 extracted from

  • Pongratz, R.L.; Kibbey, R.G.; Shulman, G.I.; Cline, G.W.
    Cytosolic and mitochondrial malic enzyme isoforms differentially control insulin secretion (2007), J. Biol. Chem., 282, 200-207.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozymes expression analysis, overview Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
additional information siRNA knockdown of cytosolic ME1 by 89% of mRNA expression and enzyme activity significantly reduces glucose and decreases the flux of both pools of pyruvate through pyruvate carboxylase affecting the anaplerotic pathways, insulin secretion in response to membrane depolarization using potassium chloride is unaffected by siRNA knockdown of malic enzyme, overview Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isozyme ME1 Rattus norvegicus 5829
-
mitochondrion isozyme ME3 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Rattus norvegicus the cycling of pyruvate by isozyme ME1 generates cytosolic NADPH pyruvate + CO2 + NADPH
-
r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
INS-1 832/13 cell
-
Rattus norvegicus
-
pancreatic beta cell isozymes ME1 and ME3 Rattus norvegicus
-
pancreatic islet
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
isozyme expression levels, pyruvate cycling and isozyme activity in pancreatic islets, overview Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Rattus norvegicus pyruvate + CO2 + NADPH
-
r
(S)-malate + NADP+ the cycling of pyruvate by isozyme ME1 generates cytosolic NADPH Rattus norvegicus pyruvate + CO2 + NADPH
-
r

Synonyms

Synonyms Comment Organism
NADP-malic enzyme
-
Rattus norvegicus
NADP-ME1
-
Rattus norvegicus
NADP-ME3
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Rattus norvegicus
NADPH
-
Rattus norvegicus