Cloned (Comment) | Organism |
---|---|
isozymes expression analysis, overview | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA knockdown of cytosolic ME1 by 89% of mRNA expression and enzyme activity significantly reduces glucose and decreases the flux of both pools of pyruvate through pyruvate carboxylase affecting the anaplerotic pathways, insulin secretion in response to membrane depolarization using potassium chloride is unaffected by siRNA knockdown of malic enzyme, overview | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isozyme ME1 | Rattus norvegicus | 5829 | - |
mitochondrion | isozyme ME3 | Rattus norvegicus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Rattus norvegicus | the cycling of pyruvate by isozyme ME1 generates cytosolic NADPH | pyruvate + CO2 + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
INS-1 832/13 cell | - |
Rattus norvegicus | - |
pancreatic beta cell | isozymes ME1 and ME3 | Rattus norvegicus | - |
pancreatic islet | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
isozyme expression levels, pyruvate cycling and isozyme activity in pancreatic islets, overview | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Rattus norvegicus | pyruvate + CO2 + NADPH | - |
r | |
(S)-malate + NADP+ | the cycling of pyruvate by isozyme ME1 generates cytosolic NADPH | Rattus norvegicus | pyruvate + CO2 + NADPH | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADP-malic enzyme | - |
Rattus norvegicus |
NADP-ME1 | - |
Rattus norvegicus |
NADP-ME3 | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |