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Literature summary for 1.1.1.40 extracted from
- The effect of NADP-dependent malic enzyme expression and anaerobic C4 metabolism in Escherichia coli compared with other anaplerotic enzymes (2007), J. Appl. Microbiol., 103, 2340-2345.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene maeB, overexpression in wild-type strain W3110, and in strains deficient for pyruvate formate lyase, lactate dehydrogenase, and glucose phosphotransferase, overview | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | effects of enzyme overexpression on the concentration of CO2 and the C4 metabolsim in wild-type strain and strains overexpressing other anaeplerotic enzymes, phenotypes, overview | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates | Escherichia coli |  |
| NH4Cl | activates | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Escherichia coli | the reaction plays a role in the C4 metabolism, pathway regulation, overview | pyruvate + CO2 + NADPH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene maeB | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01 | - |
below, wild-type strain W3110 | Escherichia coli |
| 1.69 | - |
MaeB overexpressed in an anaplerotic enzyme overexpressing strain | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Escherichia coli | pyruvate + CO2 + NADPH | - |
r | |
| (S)-malate + NADP+ | the reaction plays a role in the C4 metabolism, pathway regulation, overview | Escherichia coli | pyruvate + CO2 + NADPH | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MaeB | - |
Escherichia coli |
| NADP-dependent malic enzyme | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | specific for | Escherichia coli | |
| NADPH | specific for | Escherichia coli | |
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Release 2023.1 (January 2023)
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