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Literature summary for 1.1.1.40 extracted from

  • Mitsch, M.J.; Cowie, A.; Finan, T.M.
    Malic Enzyme Cofactor and Domain Requirements for Symbiotic N2-fixation by Sinorhizobium meliloti (2006), J. Bacteriol., 189, 160-168.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene tme, subcloning in Escherichia coli, expression in bacteroids deficient for the NAD+-dependent malic enzyme encoded by gene dme, EC 1.1.1.39 Sinorhizobium meliloti

Protein Variants

Protein Variants Comment Organism
additional information the tme gene is placed under the control of the dme promoter, and despite elevated levels of TME within bacteroids, no symbiotic nitrogen fixation occurred in dme mutant strains, the dme mutant cells fail to fix N2 in alfalfa root nodules, overview, no TME in the tme mutant RmG994 Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NADP+ Sinorhizobium meliloti
-
pyruvate + CO2 + NADPH
-
r
additional information Sinorhizobium meliloti TME is not capable of N2 fixation probably due to a constantly high ratio of NADPH + H+ to NADP+ in nitrogen-fixing bacteroids, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.008 0.89 enzyme activity in several recombinant strains, overview Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Sinorhizobium meliloti pyruvate + CO2 + NADPH
-
r
additional information TME is not capable of N2 fixation probably due to a constantly high ratio of NADPH + H+ to NADP+ in nitrogen-fixing bacteroids, overview Sinorhizobium meliloti ?
-
?

Synonyms

Synonyms Comment Organism
NADP+-dependent malic enzyme
-
Sinorhizobium meliloti
TME
-
Sinorhizobium meliloti

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Sinorhizobium meliloti
NADPH
-
Sinorhizobium meliloti