Cloned (Comment) | Organism |
---|---|
gene tme, subcloning in Escherichia coli, expression in bacteroids deficient for the NAD+-dependent malic enzyme encoded by gene dme, EC 1.1.1.39 | Sinorhizobium meliloti |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the tme gene is placed under the control of the dme promoter, and despite elevated levels of TME within bacteroids, no symbiotic nitrogen fixation occurred in dme mutant strains, the dme mutant cells fail to fix N2 in alfalfa root nodules, overview, no TME in the tme mutant RmG994 | Sinorhizobium meliloti |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Sinorhizobium meliloti | - |
pyruvate + CO2 + NADPH | - |
r | |
additional information | Sinorhizobium meliloti | TME is not capable of N2 fixation probably due to a constantly high ratio of NADPH + H+ to NADP+ in nitrogen-fixing bacteroids, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sinorhizobium meliloti | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.008 | 0.89 | enzyme activity in several recombinant strains, overview | Sinorhizobium meliloti |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Sinorhizobium meliloti | pyruvate + CO2 + NADPH | - |
r | |
additional information | TME is not capable of N2 fixation probably due to a constantly high ratio of NADPH + H+ to NADP+ in nitrogen-fixing bacteroids, overview | Sinorhizobium meliloti | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NADP+-dependent malic enzyme | - |
Sinorhizobium meliloti |
TME | - |
Sinorhizobium meliloti |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Sinorhizobium meliloti | |
NADPH | - |
Sinorhizobium meliloti |