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Literature summary for 1.1.1.40 extracted from
- Purification and physical and kinetic characterization of a photosynthetic NADP-dependent malic enzyme from the CAM plant Aptenia cordifolia (2003), Plant Sci., 164, 95-102.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | glutamate is a poor activator | Mesembryanthemum cordifolium | |
| UDP-glucose | 32% activation at 2 mM | Mesembryanthemum cordifolium |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Ketoglutarate | 34% inhibition at 2 mM | Mesembryanthemum cordifolium | |
| ADP | 83% inhibition at 2 mM | Mesembryanthemum cordifolium | |
| AMP | 75% inhibition at 2 mM | Mesembryanthemum cordifolium | |
| ATP | 83% inhibition at 2 mM | Mesembryanthemum cordifolium | |
| citrate | competitive, 61% inhibition at 5 mM | Mesembryanthemum cordifolium | |
| L-aspartate | competitive, 94% inhibition at 10 mM | Mesembryanthemum cordifolium | |
| additional information | glutamine is a poor inhibitor | Mesembryanthemum cordifolium | |
| oxaloacetate | competitive, 70% inhibition at 2 mM | Mesembryanthemum cordifolium | |
| phosphate | 35% inhibition at 5 mM | Mesembryanthemum cordifolium | |
| phosphoenolpyruvate | competitive, 82% inhibition at 2 mM | Mesembryanthemum cordifolium | |
| pyruvate | 22% inhibition at 2 mM | Mesembryanthemum cordifolium | |
| succinate | competitive, 28% inhibition at 5 mM | Mesembryanthemum cordifolium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
NADP+ | pH 7.3, 30°C, isozyme 2 | Mesembryanthemum cordifolium | |
| 1.1 | - |
L-malate | pH 7.3, 30°C, isozyme 2 | Mesembryanthemum cordifolium | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mesembryanthemum cordifolium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
4 * 72000, isozyme 2, SDS-PAGE | Mesembryanthemum cordifolium |
| 72000 | - |
x * 72000, isozyme 1, SDS-PAGE | Mesembryanthemum cordifolium |
| 264000 | - |
isozyme 2, gel filtration | Mesembryanthemum cordifolium |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | Mesembryanthemum cordifolium | no day/night regulation of the isozyme 2 in leaves via expression level but by metabolite inhibition, overview | pyruvate + CO2 + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mesembryanthemum cordifolium | - |
constitutive CAM plant, 2 isozymes | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isozyme 2, partially from leaves, 513fold | Mesembryanthemum cordifolium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | isozymes 1 and 2 | Mesembryanthemum cordifolium | - |
| root | only isozyme 1, not isozyme 2 | Mesembryanthemum cordifolium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 30.14 | - |
partially purified leaf isozyme 2 | Mesembryanthemum cordifolium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NADP+ | - |
Mesembryanthemum cordifolium | pyruvate + CO2 + NADPH | - |
? | |
| (S)-malate + NADP+ | no day/night regulation of the isozyme 2 in leaves via expression level but by metabolite inhibition, overview | Mesembryanthemum cordifolium | pyruvate + CO2 + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72000, isozyme 1, SDS-PAGE | Mesembryanthemum cordifolium |
| tetramer | 4 * 72000, isozyme 2, SDS-PAGE | Mesembryanthemum cordifolium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADP-dependent malic enzyme | - |
Mesembryanthemum cordifolium |
| NADP-ME | - |
Mesembryanthemum cordifolium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Mesembryanthemum cordifolium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
isozyme 2 | Mesembryanthemum cordifolium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | specific for, no activity with NAD+ | Mesembryanthemum cordifolium | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mesembryanthemum cordifolium | isozyme 1, isoelectric focusing | - |
6 |
| Mesembryanthemum cordifolium | isozyme 2, isoelectric focusing | - |
6 |
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