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Literature summary for 1.1.1.40 extracted from

  • Chang, G.G.; Wang, J.K.; Huang, T.M.; Lee, H.J.; Chou, W.Y.; Meng, C.L.
    Purification and characterization of the cytosolic NADP+-dependent malic enzyme from human breast cancer cell line (1991), Eur. J. Biochem., 202, 681-688.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP non-competitive versus L-malate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00139
-
NADP+
-
Homo sapiens
0.002
-
NADPH
-
Homo sapiens
0.216
-
L-malate
-
Homo sapiens
4.8
-
pyruvate
-
Homo sapiens
13.3
-
CO2
-
Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
4 * 65000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell line breast cancer cell line Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.04
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Homo sapiens pyruvate + CO2 + NADPH
-
r
pyruvate + CO2 + NADPH
-
Homo sapiens L-malate + NADP+
-
r
pyruvate + CO2 + NADPH
-
Homo sapiens (S)-malate + NADP+
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 65000, SDS-PAGE Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+ cofactor Homo sapiens
NADPH cofactor Homo sapiens