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Literature summary for 1.1.1.40 extracted from

  • Gourdon, P.; Baucher, M.F.; Lindley, N.D.; Guyonvarch, A.
    Cloning of the malic enzyme gene from Corynebacterium glutamicum and role of the enzyme in lactate metabolism (2000), Appl. Environ. Microbiol., 66, 2981-2987.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
NH4+ activates Corynebacterium glutamicum

Cloned(Commentary)

Cloned (Comment) Organism
-
Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
glutamate
-
Corynebacterium glutamicum
oxaloacetate
-
Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
NADPH
-
Corynebacterium glutamicum
0.083
-
NADP+
-
Corynebacterium glutamicum
3.8
-
L-malate
-
Corynebacterium glutamicum
13.8
-
pyruvate
-
Corynebacterium glutamicum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol represents about 1% of total cytosolic enzyme Corynebacterium glutamicum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Corynebacterium glutamicum
K+ activates Corynebacterium glutamicum
Mg2+ activates Corynebacterium glutamicum
Mn2+ dependent on presence of divalent cations. Maximal activity in presence of 5 mM Mn2+ Corynebacterium glutamicum
Ni2+ activates Corynebacterium glutamicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
2 * 42000, SDS-PAGE Corynebacterium glutamicum
105000
-
-
Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.9
-
-
Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Corynebacterium glutamicum pyruvate + CO2 + NADPH
-
r
pyruvate + CO2 + NADPH
-
Corynebacterium glutamicum L-malate + NADP+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Corynebacterium glutamicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
stable for at least 120 min Corynebacterium glutamicum
33
-
120 min, 24% loss of activity Corynebacterium glutamicum
40
-
120 min, 70% loss of activity Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
carboxylation of pyruvate Corynebacterium glutamicum
7.8
-
oxidative decarboxylation of L-malate Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
NADP+ cofactor Corynebacterium glutamicum
NADPH cofactor Corynebacterium glutamicum