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Literature summary for 1.1.1.40 extracted from
- Purification and properties of cytosolic and mitochondrial malic enzyme isolated from human brain (1995), Int. J. Biochem. Cell Biol., 27, 47-54.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0031 | - |
NADP+ | - |
Homo sapiens |  |
| 7.2 | - |
pyruvate | - |
Homo sapiens | |
| 23 | - |
CO2 | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
4 * 64000, SDS-PAGE | Homo sapiens |
| 257000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 90.9 | - |
mitochondrial enzyme | Homo sapiens |
| 91.4 | - |
cytosolic enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | lower activity than with NADP+ | Homo sapiens | pyruvate + CO2 + NADH | - |
? | |
| (S)-malate + NADP+ | - |
Homo sapiens | pyruvate + CO2 + NADPH | - |
r | |
| Oxaloacetate | - |
Homo sapiens | Pyruvate + CO2 | - |
? | |
| pyruvate + CO2 + NADPH | - |
Homo sapiens | L-malate + NADP+ | - |
r | |
| pyruvate + CO2 + NADPH | - |
Homo sapiens | (S)-malate + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 64000, SDS-PAGE | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
cytosolic enzyme, NADPH-dependent reductive carboxylation of pyruvate | Homo sapiens |
| 8 | - |
NADP+-dependent decarboxylation of malate, at 10 mM malate, mitochondrial enzyme and cytosolic enzyme | Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | pH 6.8: about 40% of maximal activity, pH 8.0: about 70% of maximal activity, cytosolic enzyme, NADPH-dependent reductive carboxylation of pyruvate | Homo sapiens |
| 7.1 | 8.6 | pH 7.1: about 65% of maximal activity, pH 8.6: about 75% of maximal activity, NADP+-dependent decarboxylation of malate, at 10 mM malate, mitochondrial enzyme and cytosolic enzyme | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | cofactor | Homo sapiens | |
| NADPH | cofactor | Homo sapiens | |
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