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Literature summary for 1.1.1.40 extracted from

  • Bukato, G.; Kochan, Z.; Swierczynski, J.
    Different regulatory properties of the cytosolic and mitochondrial forms of malic enzyme isolated from human brain (1995), Int. J. Biochem. Cell Biol., 27, 1003-1008.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
fumarate increases activity of the mitochondrial enzyme at low malate concentrations, no effect on the activity of the cytosolic enzyme Homo sapiens
succinate increases activity of the mitochondrial enzyme at low malate concentrations, no effect on the activity of the cytosolic enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
malate excess of malate inhibits the oxidative decarboxylation catalyzed by the cytosolic enzyme at pH 7.0, and below, decarboxylation catalyzed by mitochondrial enzyme is unaffected by the substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
L-malate cytosolic enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
mitochondrion
-
Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens one may speculate that in vivo the reaction catalyzed by cytosolic enzyme supplies dicarboxylic acids, anaplerotic function, for the formation of neurotransmitters while the mitochondrial enzyme regulates the flux rate via Krebs cycle by disposition of the tricarboxylic acid cycle intermediates, cataplerotic function ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Homo sapiens pyruvate + CO2 + NADPH
-
?
additional information one may speculate that in vivo the reaction catalyzed by cytosolic enzyme supplies dicarboxylic acids, anaplerotic function, for the formation of neurotransmitters while the mitochondrial enzyme regulates the flux rate via Krebs cycle by disposition of the tricarboxylic acid cycle intermediates, cataplerotic function Homo sapiens ?
-
?

Cofactor

Cofactor Comment Organism Structure
NADP+ cofactor Homo sapiens