Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.40 extracted from

  • Bartholome, K.; Brdiczka, D.G.; Pette, D.
    Purification and properties of extra- and intramitochondrial malate dehydrogenase (NADP; decarboxylating) from pig heart (1972), Hoppe-Seyler's Z. Physiol. Chem., 353, 1487-1495.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA inhibition is much more pronounced in the mitochondrial enzyme than in the cytosolic enzyme and occurs at physiological acetyl-CoA concentrations Sus scrofa
malonyl-CoA inhibition of mitochondrial enzyme and cytosolic enzyme to a much lower extent than with acetyl-CoA Sus scrofa
Mg2+ mitochondrial enzyme, decarboxylation reaction, above 6 mM Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00118
-
NADP+ cytosolic enzyme Sus scrofa
0.00188
-
NADP+ mitochondrial enzyme Sus scrofa
0.14
-
L-malate cytosolic enzyme Sus scrofa
1.25
-
L-malate mitochondrial enzyme Sus scrofa
10
-
pyruvate cytosolic enzyme Sus scrofa
25
-
pyruvate mitochondrial enzyme Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol 2 distinct enzyme forms are present in cytosol and in mitochondria Sus scrofa 5829
-
mitochondrion 2 distinct enzyme forms are present in cytosol and in mitochondria Sus scrofa 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity of cytosolic enzyme and mitochondrial enzyme is strictly dependent on Sus scrofa
Mg2+ optimal concentration: 6 mM for the decarboxylation of the mitochondrial enzyme, 20 mM for the cytosolic enzyme Sus scrofa
Mn2+ activity of cytosolic enzyme and mitochondrial enzyme is strictly dependent on Sus scrofa
Mn2+ optimal concentration is 15 mM for the cytosolic enzyme and 10 mM for the mitochondrial enzyme Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
216000
-
cytosolic enzyme, density gradient centrifugation Sus scrofa
227000
-
mitochondrial enzyme, density gradient centrifugation Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 distinct enzyme forms Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
cardiac muscle
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Sus scrofa pyruvate + CO2 + NADPH
-
r
pyruvate + CO2 + NADPH
-
Sus scrofa L-malate + NADP+
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6
-
mitochondrial enzyme, carboxylation Sus scrofa
7
-
carboxylation and decarboxylation, cytosolic enzyme Sus scrofa
7.6
-
cytosolic enzyme, decarboxylation Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADP+ cofactor Sus scrofa
NADPH cofactor Sus scrofa