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Literature summary for 1.1.1.4 extracted from
- Purification, characterization and some properties of diacetyl(acetoin) reductase from Enterobacter aerogenes (1991), Eur. J. Biochem., 198, 327-332.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
NAD+ | - |
Klebsiella aerogenes |  |
| 0.4 | - |
acetoin | - |
Klebsiella aerogenes | |
| 1.6 | - |
diacetyl | - |
Klebsiella aerogenes | |
| 2.6 | - |
2,3-Butanediol | - |
Klebsiella aerogenes | |
| 6 | - |
2,3-pentanediol | - |
Klebsiella aerogenes | |
| 18 | - |
Methyl pyruvate | - |
Klebsiella aerogenes | |
| 20 | - |
ethyl pyruvate | - |
Klebsiella aerogenes | |
| 75 | - |
methyl glyoxal | - |
Klebsiella aerogenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
2 * 28000, SDS-PAGE | Klebsiella aerogenes |
| 61000 | - |
gel filtration | Klebsiella aerogenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella aerogenes | - |
authors suggest systematic name: -glycol:NAD+ oxidoreductase ??? | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Klebsiella aerogenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 270 | - |
- |
Klebsiella aerogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-butanediol + NAD+ | - |
Klebsiella aerogenes | acetoin + NADH | - |
r | |
| 2,3-pentanediol + NAD+ | - |
Klebsiella aerogenes | ? | - |
r | |
| acetoin + NADH + H+ | - |
Klebsiella aerogenes | 2,3-butanediol + NAD+ | - |
r | |
| diacetyl + NADH | - |
Klebsiella aerogenes | 2,3-butanediol + NAD+ | - |
? | |
| ethyl pyruvate + NADH | - |
Klebsiella aerogenes | ? | - |
? | |
| methyl glyoxal + NADH | - |
Klebsiella aerogenes | ? | - |
? | |
| methyl pyruvate + NADH | - |
Klebsiella aerogenes | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 28000, SDS-PAGE | Klebsiella aerogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Klebsiella aerogenes |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | no activity with NADP+ as cofactor | Klebsiella aerogenes | |
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Release 2023.1 (January 2023)
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