Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | [Clostridium] scindens |
Crystallization (Comment) | Organism |
---|---|
apoenzyme isoform BaiA2 and in complex with NAD+, sitting nano-drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.1M Tris-HCl pH 8.5, and 0.2 M sodium acetate | [Clostridium] scindens |
Protein Variants | Comment | Organism |
---|---|---|
E42A | the mutation improves specificity toward NADP+ by 10fold compared to the wild type enzyme | [Clostridium] scindens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.0013 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.0015 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.0018 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.0033 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.0056 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.0067 | - |
NAD+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.0083 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.0091 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.015 | - |
NADP+ | mutant E42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.016 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.017 | - |
NAD+ | mutant E42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.017 | - |
NAD+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.017 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.018 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.022 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.023 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.032 | - |
choloyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.053 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.084 | - |
NADP+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.106 | - |
choloyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.201 | - |
taurocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.205 | - |
glycocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.329 | - |
taurocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.334 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.383 | - |
glycocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.555 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.802 | - |
cholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.883 | - |
NADP+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.158 | - |
cholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] scindens | P19337 | - |
- |
[Clostridium] scindens VPI 12708 | P19337 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | [Clostridium] scindens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-oxochenodeoxycholoyl-CoA + NAD+ | - |
[Clostridium] scindens | ? + NADH + H+ | - |
? | |
3-oxochenodeoxycholoyl-CoA + NAD+ | - |
[Clostridium] scindens VPI 12708 | ? + NADH + H+ | - |
? | |
3-oxocholoyl CoA + NAD+ | - |
[Clostridium] scindens | ? + NADH + H+ | - |
? | |
3-oxocholoyl CoA + NAD+ | - |
[Clostridium] scindens VPI 12708 | ? + NADH + H+ | - |
? | |
3-oxodeoxycholoyl-CoA + NAD+ | - |
[Clostridium] scindens | ? + NADH + H+ | - |
? | |
3-oxodeoxycholoyl-CoA + NAD+ | - |
[Clostridium] scindens VPI 12708 | ? + NADH + H+ | - |
? | |
3-oxolithocholoyl-CoA + NAD+ | - |
[Clostridium] scindens | ? + NADH + H+ | - |
? | |
3-oxolithocholoyl-CoA + NAD+ | - |
[Clostridium] scindens VPI 12708 | ? + NADH + H+ | - |
? | |
chenodeoxycholic acid + NAD+ | - |
[Clostridium] scindens | 7-alpha-hydroxy-3-oxo-5beta-cholanate + NADH + H+ | - |
? | |
chenodeoxycholic acid + NAD+ | - |
[Clostridium] scindens VPI 12708 | 7-alpha-hydroxy-3-oxo-5beta-cholanate + NADH + H+ | - |
? | |
chenodeoxycholoyl-CoA + NADP+ | - |
[Clostridium] scindens | 3-oxochenodeoxycholoyl-CoA + NADPH + H+ | - |
? | |
chenodeoxycholyl CoA + NAD+ | - |
[Clostridium] scindens | 3-oxo-chenodeoxycholy-CoA + NADH + H+ | - |
? | |
cholic acid + NAD+ | - |
[Clostridium] scindens | 3-oxocholic acid + NADH + H+ | - |
? | |
choloyl-CoA + NAD+ | - |
[Clostridium] scindens | 3-oxocholoyl-CoA + NADH + H+ | - |
? | |
deoxycholoyl-CoA + NAD+ | - |
[Clostridium] scindens | 3-oxodeoxycholoyl-CoA + NADH + H+ | - |
? | |
glycocholic acid + NAD+ | - |
[Clostridium] scindens | N-[7alpha,12-alpha-3,24-dioxo-5beta-cholan-24-yl]glycine + NADH + H+ | - |
? | |
lithocholoyl-CoA + NAD+ | - |
[Clostridium] scindens | 3-oxolithocholoyl-CoA + NADH + H+ | - |
? | |
taurocholic acid + NAD+ | - |
[Clostridium] scindens | 3-oxotaurocholic acid + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3alpha-hydroxy bile acid-CoA-ester 3-dehydrogenase | - |
[Clostridium] scindens |
baiA1 | isoform | [Clostridium] scindens |
baiA2 | isoform | [Clostridium] scindens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
NADP+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.05 | - |
NADP+ | mutantE42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.055 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.089 | - |
NAD+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.09 | - |
cholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.11 | - |
NADP+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.12 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.14 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.145 | - |
glycocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.15 | - |
taurocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.15 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.16 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.17 | - |
choloyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.17 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.18 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.2 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.25 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.27 | - |
cholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.3 | - |
NAD+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.32 | - |
NAD+ | mutant E42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.4 | - |
taurocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.4 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.42 | - |
glycocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.55 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.7 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.73 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.73 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.82 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.87 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1.23 | - |
choloyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preferred cofactor | [Clostridium] scindens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
cholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.13 | - |
NADP+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.17 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.23 | - |
cholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.3 | - |
NADP+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.45 | - |
chenodeoxycholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.72 | - |
glycocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
0.73 | - |
taurocholic acid | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
1 | - |
taurocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
1 | - |
glycocholic acid | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
3.3 | - |
NADP+ | mutantE42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
5.22 | - |
choloyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
8.7 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
9.32 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
10.18 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
11.6 | - |
choloyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
13.17 | - |
NAD+ | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
15.4 | - |
chenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
17.7 | - |
NAD+ | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
18.5 | - |
NAD+ | mutant E42A of isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
33.3 | - |
lithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
54.2 | - |
deoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
60.4 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
76.85 | - |
3-oxocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
84.3 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
93.58 | - |
3-oxolithocholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
101.7 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens | |
120.5 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
131 | - |
3-oxodeoxycholoyl-CoA | wild type enzyme isoform BaiA1, at pH 8.7 and 37°C | [Clostridium] scindens | |
151.5 | - |
3-oxochenodeoxycholoyl-CoA | wild type enzyme isoform BaiA2, at pH 8.7 and 37°C | [Clostridium] scindens |