Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.39 extracted from

  • Canellas, P.F.; Wedding, R.T.
    Kinetic properties of NAD malic enzyme from cauliflower (1984), Arch. Biochem. Biophys., 229, 414-425.
    View publication on PubMed
Search for more literature for 1.1.1.39

Inhibitors

Inhibitors Comment Organism Structure
KCNO
-
 Brassica oleracea
NEM
-
 Brassica oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
 NAD+ with 30 mM Mg2+ Brassica oleracea
0.48
-
 NAD+ with 8 mM Mg2+ Brassica oleracea
0.5
-
 NAD+ with 80 mM Mg2+ Brassica oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can replace Mg2+ in activation. Km: 0.018 mM Brassica oleracea
Mg2+ no decarboxylation of malate in absence of either Mg2+ or NAD+. Km: 0.04 mM Brassica oleracea
Mn2+ can replace Mg2+ in activation Brassica oleracea

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
 var. botrytis
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brassica oleracea

Reaction

Reaction Comment Organism Reaction ID
(S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ sequential mechanism, each of the substrate pairs binds randomly to the enzyme Brassica oleracea
a

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
 Brassica oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malate + NAD+ no decarboxylation of malate in absence of either Mg2+ or NAD+ Brassica oleracea pyruvate + CO2 + NADH + H+
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+ cofactor Brassica oleracea