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Literature summary for 1.1.1.386 extracted from

  • Figueroa-Teran, R.; Welch, W.H.; Blomquist, G.J.; Tittiger, C.
    Ipsdienol dehydrogenase (IDOLDH): a novel oxidoreductase important for Ips pini pheromone production (2012), Insect Biochem. Mol. Biol., 42, 81-90.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
the recombinant enzyme is produced in Sf9 cells using a baculovirus expression system Ips pini

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
(4R)-ipsdienol pH 7.8, 25°C Ips pini

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Ips pini 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
calculated from sequence Ips pini

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(4R)-ipsdienol + NAD(P)+ Ips pini the enzyme is involved in pheromon production ipsdienone + NAD(P)H + H+
-
?

Organism

Organism UniProt Comment Textmining
Ips pini G5DGA8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
midgut transcript levels are highest in pheromone producing tissue: the anterior midgut of fed males. The protein is detected only in male midguts Ips pini
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0163
-
pH 7.8, 25°C Ips pini

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(4R)-ipsdienol + NAD(P)+ the enzyme is involved in pheromon production Ips pini ipsdienone + NAD(P)H + H+
-
?
(4R)-ipsdienol + NAD+ no activity with (4S)-ipsdienol. The enzyme shows similar specific activities with NAD+ and NADP+ Ips pini ipsdienone + NADH + H+
-
?
(4R)-ipsdienol + NADP+ no activity with (4S)-ipsdienol. The enzyme shows similar specific activities with NAD+ and NADP+ Ips pini ipsdienone + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
IDOLDH
-
Ips pini

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Ips pini

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Ips pini

Cofactor

Cofactor Comment Organism Structure
NAD+ the enzyme shows similar specific activities with NAD+ and NADP+ Ips pini
NADP+ the enzyme shows similar specific activities with NAD+ and NADP+ Ips pini

pI Value

Organism Comment pI Value Maximum pI Value
Ips pini calculated from sequence
-
5.8

General Information

General Information Comment Organism
metabolism the enzyme is involved in pheromon production Ips pini