Literature summary for 1.1.1.383 extracted from

Milne, N.; Wahl, S.; van Maris, A.; Pronk, J.; Daran, J.
Excessive by-product formation: A key contributor to low isobutanol yields of engineered (2016), Metab. Eng. Commun., 3, 39-51.

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Application

Application	Comment	Organism
biofuel production	a cytosolically located, cofactor-balanced isobutanol pathway, consisting of a mosaic of bacterial enzymes is expressed in Sacchaormyces cerevisiae. In aerobic cultures, the pathway intermediate isobutyraldehyde is oxidized to isobutyrate rather than reduced to isobutanol. Significant concentrations of the pathway intermediates 2,3-dihydroxyisovalerate and alpha-ketoisovalerate, as well as diacetyl and acetoin, accumulate extracellularly. While the engineered strain cannot grow anaerobically, micro-aerobic cultivation results in isobutanol formation at a yield of 0.018 mol/mol glucose. Simultaneously, 2,3-butanediol is produced at a yield of 0.64 mol/molglucose	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P05793	-	-

Synonyms

Synonyms	Comment	Organism
ilvC	-	Escherichia coli

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Release 2023.1 (January 2023)