Any feedback?
Literature summary for 1.1.1.381 extracted from
- Characterization of short-chain dehydrogenase/reductase homologues of Escherichia coli (YdfG) and Saccharomyces cerevisiae (YMR226C) (2003), Biochim. Biophys. Acta, 1645, 89-94.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli JM109 | Escherichia coli |
| expression in Escherichia coli JM109 | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
NADP+ | pH 9.0, 30°C | Escherichia coli |  |
| 3 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 7 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 29 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
| 33 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
| 36 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 40 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
| 50 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
| 57 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 60 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
| 60 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 61 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 69 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
| 71 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 95 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 95 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P39831 | - |
- |
| Saccharomyces cerevisiae | Q05016 | - |
- |
| Saccharomyces cerevisiae ATCC 204508 | Q05016 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.4 | - |
pH 9.0, 30°C | Escherichia coli |
| 6 | - |
pH 9.0, 30°C | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 31% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| D-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| D-glycerate + NADP+ | the Vmax/KM value is 20% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| D-serine + NADP+ | the Vmax/KM value is 15% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| D-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| D-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
| D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| D-threonine + NADP+ | the Vmax/KM value is 55% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
| L-3-hydroxybutyrate + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 34% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 3.8% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| L-3-hydroxyisobutyrate + NADP+ | the Vmax/KM value is 3.8% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
| L-allo-threonine + NADP+ | highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-ketobutyrate, which is spontaneously decarboxylated into aminoacetone | Escherichia coli | aminoacetone + CO2 + NADPH + H+ | - |
? | |
| L-allo-threonine + NADP+ | highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-oxobutyrate, which is spontaneously decarboxylated into aminoacetone | Saccharomyces cerevisiae | aminoacetone + CO2 + NADPH + H+ | - |
? | |
| L-allo-threonine + NADP+ | highest Vmax/Km value of all substrates tested. The hydroxyl group of L-allo-threonine is oxidized by the enzymes to yield L-2-amino-3-oxobutyrate, which is spontaneously decarboxylated into aminoacetone | Saccharomyces cerevisiae ATCC 204508 | aminoacetone + CO2 + NADPH + H+ | - |
? | |
| L-glycerate + NADP+ | the Vmax/KM value is 18% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| L-serine + NADP+ | the Vmax/KM value is 53% compared to L-allo-threonine | Escherichia coli | ? | - |
? | |
| L-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae | ? | - |
? | |
| L-serine + NADP+ | the Vmax/KM value is less than 1% compared to L-allo-threonine | Saccharomyces cerevisiae ATCC 204508 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ydfG | - |
Escherichia coli |
| YMR226c | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
| 30 | - |
assay at | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
when heated for 10 min in 0.1 M potassium phosphate buffer (pH 7.4) containing 0.01% 2-mercaptoethanol and 10% glycerol, the enzyme is stable at up to 40°C | Saccharomyces cerevisiae |
| 55 | - |
when heated for 10 min in 0.1 M potassium phosphate buffer (pH 7.4) containing 0.01% 2-mercaptoethanol and 10% glycerol, the enzyme is stable at up to 55°C | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.17 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
| 2.01 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
| 3.5 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
| 3.78 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 4.03 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 4.2 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
| 5.76 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
| 6.55 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
| 7.84 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 8.61 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 9.38 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 11 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 20.1 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 43.8 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 52.8 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
oxidation of L-serine | Escherichia coli |
| 8.5 | - |
oxidation of L-serine | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | most stable over the pH-range 6.5 to 10.0 | Escherichia coli |
| 7.5 | 10.5 | most stable over the pH-range 6.5 to 10.0 | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | no activity with NAD+ | Escherichia coli | |
| NADP+ | no activity with NAD+ | Saccharomyces cerevisiae | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
L-Glycerate | pH 9.0, 30°C | Escherichia coli | |
| 0.04 | - |
D-glycerate | pH 9.0, 30°C | Escherichia coli | |
| 0.05 | - |
D-serine | pH 9.0, 30°C | Escherichia coli | |
| 0.062 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 0.067 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Escherichia coli | |
| 0.099 | - |
L-3-hydroxybutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 0.105 | - |
L-serine | pH 9.0, 30°C | Escherichia coli | |
| 0.109 | - |
D-threonine | pH 9.0, 30°C | Escherichia coli | |
| 0.11 | - |
D-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 0.116 | - |
L-serine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 0.151 | - |
D-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 0.199 | - |
L-allo-threonine | pH 9.0, 30°C | Escherichia coli | |
| 0.558 | - |
L-3-hydroxyisobutyrate | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 7.5 | - |
D-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
| 14.6 | - |
L-allo-threonine | pH 9.0, 30°C | Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2026.1 (March 2026)
Legal
Curated at
Member of
![DSMZ Digital Diversity]()
![Global Core Biodata Resource]()
![ELIXIR Core Data Resource]()
![German Network for Bioinformatics Infrastructure]()
