Protein Variants | Comment | Organism |
---|---|---|
L310R | the mutant strongly prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
L310R/Q401C | the mutant prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
L310R/Q401C | the mutant strongly prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
L310R/R346A/Q401C | the mutant prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
L310R/R346D/Q401C | the mutant prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
L310R/R346F/Q401C | the mutant strongly prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
L310R/R346K/Q401C | the mutant strongly prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
L310R/R346Y/Q401C | the mutant strongly prefers nicotinamide cytosine dinucleotide as cofactor over NAD+ | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
NAD+ | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.13 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.27 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
0.29 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.44 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.76 | - |
NAD+ | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
1.11 | - |
NAD+ | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
2.31 | - |
NAD+ | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
4.83 | - |
NAD+ | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
5 | - |
NAD+ | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
5 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
5.32 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
9.4 | - |
nicotinamide cytosine dinucleotide | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
10.24 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
17.2 | - |
NAD+ | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
102 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Escherichia coli | - |
pyruvate + CO2 + NADH | - |
r | |
pyruvate + CO2 + NADH | Escherichia coli | - |
(S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P26616 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Escherichia coli | pyruvate + CO2 + NADH | - |
r | |
(S)-malate + NAD+ | - |
Escherichia coli | pyruvate + CO2 + NADH + H+ | - |
r | |
(S)-malate + nicotinamide cytosine dinucleotide | - |
Escherichia coli | ? | - |
r | |
pyruvate + CO2 + NADH | - |
Escherichia coli | (S)-malate + NAD+ | - |
r | |
pyruvate + CO2 + NADH + H+ | - |
Escherichia coli | (S)-malate + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
malic enzyme | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NAD+ | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.02 | - |
NAD+ | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.08 | - |
NAD+ | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.08 | - |
NAD+ | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.12 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.18 | - |
NAD+ | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.6 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
0.64 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.86 | - |
NAD+ | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
1.53 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
2.69 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
3.38 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
5.6 | - |
NAD+ | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
9.33 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
26.1 | - |
nicotinamide cytosine dinucleotide | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
57.3 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
nicotinamide cytosine dinucleotide | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.02 | - |
NAD+ | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.03 | - |
NAD+ | mutant enzyme L310R/R346D/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.05 | - |
NAD+ | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.07 | - |
NAD+ | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.08 | - |
NAD+ | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.22 | - |
NAD+ | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.31 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346A/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
0.9 | - |
NAD+ | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
0.91 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346F/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
2.21 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346Y/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
2.8 | - |
nicotinamide cytosine dinucleotide | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli | |
7.68 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/R346K/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
20.69 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R/Q401C, pH and temperature not specified in the publication | Escherichia coli | |
169.9 | - |
nicotinamide cytosine dinucleotide | mutant enzyme L310R, pH and temperature not specified in the publication | Escherichia coli | |
214.8 | - |
NAD+ | wild type enzyme, pH and temperature not specified in the publication | Escherichia coli |