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Literature summary for 1.1.1.38 extracted from

  • Yamaguchi, M.; Tokushige, M.; Takeo, K.; Katsuki, H.
    Studies on the regulatory functions of malic enzymes: III. Regulatory effects of L-aspartate, Coenzyme A, and divalent metal ions on NAD-linked malic enzyme from Escherichia coli (1974), J. Biochem., 76, 1259-1268.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
alpha-methyl-DL-aspartate 1.35fold activation Escherichia coli W
beta-methyl-DL-aspartate 1.34fold activation Escherichia coli W
D-Aspartate 2 mM, 1.27fold activation Escherichia coli W
L-aspartate 2 mM, 1.35fold activation Escherichia coli W

Inhibitors

Inhibitors Comment Organism Structure
CoA in the absence of L-aspartate 50% inhibition with 0.22 mM CoA, in the presence of 2 mM L-aspartate 50% inhibition with 1.1 mM Escherichia coli W

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.046
-
NAD+ in the presence of 5 mM L-aspartate Escherichia coli W
0.058
-
NAD+ in the presence of 0.5 mM L-aspartate Escherichia coli W
0.071
-
NAD+ nearly hyperbolic NAD+ saturation curve Escherichia coli W
0.19
-
L-malate in the presence of 1 mM L-aspartate, pH 7.9 Escherichia coli W
0.22
-
L-malate in the presence of 2 mM L-aspartate, pH 5.5 Escherichia coli W
0.26
-
L-malate in the absence of L-aspartate, pH 5.5 Escherichia coli W
0.4
-
L-malate in the absence of L-aspartate, pH 7.9 Escherichia coli W
0.58
-
L-malate in the presence of 2 mM L-aspartate, pH 8.5 Escherichia coli W
1.4
-
L-malate in the absence of L-aspartate, pH 8.5 Escherichia coli W

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Escherichia coli W
-
CO2 + pyruvate + NADH
-
?
Oxaloacetate Escherichia coli W
-
CO2 + pyruvate
-
ir

Organism

Organism UniProt Comment Textmining
Escherichia coli W
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Escherichia coli W CO2 + pyruvate + NADH
-
?
(S)-malate + NAD+
-
Escherichia coli W CO2 + pyruvate + NADH
-
r
Oxaloacetate
-
Escherichia coli W CO2 + pyruvate
-
ir

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
22% activity left after 4 min, 80% activity left in the presence of 10 mM L-aspartate and 1 mM MnCl2, 78% activity left in the presence of 0.7 mM CoA Escherichia coli W

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
0.3 mM L-malate Escherichia coli W
7.6
-
0.3 mM L-malate, in the presence of 2 mM L-aspartate Escherichia coli W

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli W