Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-methyl-DL-aspartate | 1.35fold activation | Escherichia coli W | |
beta-methyl-DL-aspartate | 1.34fold activation | Escherichia coli W | |
D-Aspartate | 2 mM, 1.27fold activation | Escherichia coli W | |
L-aspartate | 2 mM, 1.35fold activation | Escherichia coli W |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoA | in the absence of L-aspartate 50% inhibition with 0.22 mM CoA, in the presence of 2 mM L-aspartate 50% inhibition with 1.1 mM | Escherichia coli W |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
NAD+ | in the presence of 5 mM L-aspartate | Escherichia coli W | |
0.058 | - |
NAD+ | in the presence of 0.5 mM L-aspartate | Escherichia coli W | |
0.071 | - |
NAD+ | nearly hyperbolic NAD+ saturation curve | Escherichia coli W | |
0.19 | - |
L-malate | in the presence of 1 mM L-aspartate, pH 7.9 | Escherichia coli W | |
0.22 | - |
L-malate | in the presence of 2 mM L-aspartate, pH 5.5 | Escherichia coli W | |
0.26 | - |
L-malate | in the absence of L-aspartate, pH 5.5 | Escherichia coli W | |
0.4 | - |
L-malate | in the absence of L-aspartate, pH 7.9 | Escherichia coli W | |
0.58 | - |
L-malate | in the presence of 2 mM L-aspartate, pH 8.5 | Escherichia coli W | |
1.4 | - |
L-malate | in the absence of L-aspartate, pH 8.5 | Escherichia coli W |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Escherichia coli W | - |
CO2 + pyruvate + NADH | - |
? | |
Oxaloacetate | Escherichia coli W | - |
CO2 + pyruvate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli W | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Escherichia coli W | CO2 + pyruvate + NADH | - |
? | |
(S)-malate + NAD+ | - |
Escherichia coli W | CO2 + pyruvate + NADH | - |
r | |
Oxaloacetate | - |
Escherichia coli W | CO2 + pyruvate | - |
ir |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
22% activity left after 4 min, 80% activity left in the presence of 10 mM L-aspartate and 1 mM MnCl2, 78% activity left in the presence of 0.7 mM CoA | Escherichia coli W |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
0.3 mM L-malate | Escherichia coli W |
7.6 | - |
0.3 mM L-malate, in the presence of 2 mM L-aspartate | Escherichia coli W |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli W |