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Literature summary for 1.1.1.38 extracted from

  • Driscoll, B.T.; Finan, T.M.
    Properties of NAD+ and NADP+-depemndent malic enzymes of Rhizobium (Sinorhizobium) meliloti and differential expression of their genes in nitrogen-fixing bacteroids (1997), Microbiology, 143, 489-498.
    View publication on PubMed
Search for more literature for 1.1.1.38

Activating Compound

Activating Compound Comment Organism Structure
NH4+ 30 mM Na+L-malate instead of K+L-malate reduces enzyme activity to 18% in the presence of 3 mM MnCl2, activity is restored with 5 or 50 mM NH4Cl Sinorhizobium meliloti

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli Sinorhizobium meliloti

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA 0.005 mM, 18% inhibition, 0.01 mM, 42% inhibition, 0.1 mM, 68% inhibition Sinorhizobium meliloti
Ca2+
-
 Sinorhizobium meliloti
Cu2+
-
 Sinorhizobium meliloti
Zn2+ 5 mM ZnCl2 Sinorhizobium meliloti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.142
-
 NAD+
-
 Sinorhizobium meliloti
8.6
-
 L-malate
-
 Sinorhizobium meliloti

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ at 0.002 mM MgCl2 enzyme activity is reduced to 25% without additional divalent cations, activity is restored with 0.5 or 5.0 mM CoCl2 Sinorhizobium meliloti
Cs+ 30 mM Na+L-malate instead of K+L-malate reduces enzyme activity to 18% in the presence of 3 mM MnCl2, activity is restored with 5 or 50 mM CsCl Sinorhizobium meliloti
K+ 30 mM Na+L-malate instead of K+L-malate reduces enzyme activity to 18% in the presence of 3 mM MnCl2, activity is restored with 50 mM KCl Sinorhizobium meliloti
Mg2+ at 0.002 mM MgCl2 enzyme activity is reduced to 25% without additional divalent cations, activity is restored with 0.5 or 5.0 mM MgCl2 Sinorhizobium meliloti
Mn2+ at 0.002 mM MgCl2 enzyme activity is reduced to 25% without additional divalent cations, activity is restored with 0.5 or 5.0 mM MnCl2 Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Sinorhizobium meliloti
-
 CO2 + pyruvate + NADH
-
 ?
(S)-malate + NADP+ Sinorhizobium meliloti 20% activity compared to cofactor NAD+ CO2 + pyruvate + NADPH
-
 ?
Oxaloacetate Sinorhizobium meliloti
-
 CO2 + pyruvate
-
 ir

Organism

Organism UniProt Comment Textmining
Sinorhizobium meliloti
-
 now Sinorhizobium meliloti
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.545
-
-
 Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Sinorhizobium meliloti CO2 + pyruvate + NADH
-
 ?
(S)-malate + NAD+
-
 Sinorhizobium meliloti CO2 + pyruvate + NADH
-
 r
(S)-malate + NADP+ 20% activity compared to cofactor NAD+ Sinorhizobium meliloti CO2 + pyruvate + NADPH
-
 ?
Oxaloacetate
-
 Sinorhizobium meliloti CO2 + pyruvate
-
 ir

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Sinorhizobium meliloti

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Sinorhizobium meliloti
NADP+
-
 Sinorhizobium meliloti