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Literature summary for 1.1.1.378 extracted from

  • Watanabe, S.; Saimura, M.; Makino, K.
    Eukaryotic and bacterial gene clusters related to an alternative pathway of non-phosphorylated L-rhamnose metabolism (2008), J. Biol. Chem., 283, 20372-20382.
    View publication on PubMed
Search for more literature for 1.1.1.378

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Azotobacter vinelandii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.34
-
 L-rhamnose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
2.61
-
 L-rhamnose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
4.96
-
 L-Lyxose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
8.43
-
 L-Lyxose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
89.3
-
 L-Mannose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
108
-
 L-Mannose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
582
-
 L-fucose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
673
-
 L-fucose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-rhamnose + NAD(P)+ Azotobacter vinelandii first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway L-rhamno-1,4-lactone + NAD(P)H + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Azotobacter vinelandii
-
-
-
Azotobacter vinelandii NBRC 102612
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azotobacter vinelandii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
 pH 9.0, 30°C, substrates: L-fucose, NAD+ Azotobacter vinelandii
0.27
-
 pH 9.0, 30°C, substrates: L-fucose, NADP+ Azotobacter vinelandii
1.69
-
 pH 9.0, 30°C, substrates: L-mannose, NAD+ Azotobacter vinelandii
3.64
-
 pH 9.0, 30°C, substrates: L-mannose, NADP+ Azotobacter vinelandii
50.7
-
 pH 9.0, 30°C, substrates: L-lyxose, NAD+ Azotobacter vinelandii
72.1
-
 pH 9.0, 30°C, substrates: L-rhamnose, NAD+ Azotobacter vinelandii
125
-
 pH 9.0, 30°C, substrates: L-lyxose, NADP+ Azotobacter vinelandii
163
-
 pH 9.0, 30°C, substrates: L-rhamnose, NADP+ Azotobacter vinelandii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-fucose + NAD+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
 ?
L-fucose + NADP+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
 ?
L-lyxose + NAD+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
 ?
L-lyxose + NADP+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
 ?
L-mannose + NAD+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
 ?
L-mannose + NADP+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii ?
-
 ?
L-rhamnose + NAD(P)+ first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway Azotobacter vinelandii L-rhamno-1,4-lactone + NAD(P)H + H+
-
 ?
L-rhamnose + NAD+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii L-rhamno-1,4-lactone + NADH + H+
-
 ?
L-rhamnose + NADP+ the enzyme shows significant preference for NADP+ over NAD+ Azotobacter vinelandii L-rhamno-1,4-lactone + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
AvLRA1
-
 Azotobacter vinelandii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Azotobacter vinelandii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.8
-
 L-fucose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
9.2
-
 L-fucose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
9.4
-
 L-Mannose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
17
-
 L-Mannose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
37.2
-
 L-rhamnose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
41.3
-
 L-Lyxose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
74.8
-
 L-rhamnose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
79.7
-
 L-Lyxose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Azotobacter vinelandii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Azotobacter vinelandii
NADP+
-
 Azotobacter vinelandii

General Information

General Information Comment Organism
physiological function first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway Azotobacter vinelandii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.007
-
 L-fucose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
0.016
-
 L-fucose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
0.09
-
 L-Mannose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
0.19
-
 L-Mannose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
4.9
-
 L-Lyxose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
14.3
-
 L-rhamnose pH 9.0, 30°C, cosubstrate: NAD+ Azotobacter vinelandii
16.05
-
 L-Lyxose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii
35.7
-
 L-rhamnose pH 9.0, 30°C, cosubstrate: NADP+ Azotobacter vinelandii