Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.34 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
2.61 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
4.96 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
8.43 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
89.3 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
108 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
582 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
673 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-rhamnose + NAD(P)+ | Azotobacter vinelandii | first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway | L-rhamno-1,4-lactone + NAD(P)H + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Azotobacter vinelandii NBRC 102612 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Azotobacter vinelandii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.15 | - |
pH 9.0, 30°C, substrates: L-fucose, NAD+ | Azotobacter vinelandii |
0.27 | - |
pH 9.0, 30°C, substrates: L-fucose, NADP+ | Azotobacter vinelandii |
1.69 | - |
pH 9.0, 30°C, substrates: L-mannose, NAD+ | Azotobacter vinelandii |
3.64 | - |
pH 9.0, 30°C, substrates: L-mannose, NADP+ | Azotobacter vinelandii |
50.7 | - |
pH 9.0, 30°C, substrates: L-lyxose, NAD+ | Azotobacter vinelandii |
72.1 | - |
pH 9.0, 30°C, substrates: L-rhamnose, NAD+ | Azotobacter vinelandii |
125 | - |
pH 9.0, 30°C, substrates: L-lyxose, NADP+ | Azotobacter vinelandii |
163 | - |
pH 9.0, 30°C, substrates: L-rhamnose, NADP+ | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-fucose + NAD+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
L-fucose + NADP+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
L-lyxose + NAD+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
L-lyxose + NADP+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
L-mannose + NAD+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
L-mannose + NADP+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | ? | - |
? | |
L-rhamnose + NAD(P)+ | first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway | Azotobacter vinelandii | L-rhamno-1,4-lactone + NAD(P)H + H+ | - |
? | |
L-rhamnose + NAD+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | L-rhamno-1,4-lactone + NADH + H+ | - |
? | |
L-rhamnose + NADP+ | the enzyme shows significant preference for NADP+ over NAD+ | Azotobacter vinelandii | L-rhamno-1,4-lactone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AvLRA1 | - |
Azotobacter vinelandii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Azotobacter vinelandii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
9.2 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
9.4 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
17 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
37.2 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
41.3 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
74.8 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
79.7 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Azotobacter vinelandii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Azotobacter vinelandii | |
NADP+ | - |
Azotobacter vinelandii |
General Information | Comment | Organism |
---|---|---|
physiological function | first enzyme of the non-phosphorylative L-rhamnose pathway in archaea, that is analogous to the Entner-Doudoroff pathway | Azotobacter vinelandii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
0.016 | - |
L-fucose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
0.09 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
0.19 | - |
L-Mannose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
4.9 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
14.3 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NAD+ | Azotobacter vinelandii | |
16.05 | - |
L-Lyxose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii | |
35.7 | - |
L-rhamnose | pH 9.0, 30°C, cosubstrate: NADP+ | Azotobacter vinelandii |