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Literature summary for 1.1.1.376 extracted from

  • Watanabe, Y.; Iga, C.; Watanabe, Y.; Watanabe, S.
    Structural insights into the catalytic and substrate recognition mechanisms of bacterial L-arabinose 1-dehydrogenase (2019), FEBS Lett., 593, 1257-1266 .
    View publication on PubMed
Search for more literature for 1.1.1.376

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Azospirillum brasilense

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapor diffusion method at 20°C, crystal structures of apo- and NADP-bound AraDH at 1.5 and 2.2 A resolutions Azospirillum brasilense

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 NADP+ pH 9.0, 30°C Azospirillum brasilense
1.586
-
 NAD+ pH 9.0, 30°C Azospirillum brasilense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose + NAD+ Azospirillum brasilense
-
 L-arabinono-1,4-lactone + NADH + H+
-
 ?
L-arabinose + NAD+ Azospirillum brasilense ATCC29145
-
 L-arabinono-1,4-lactone + NADH + H+
-
 ?
L-arabinose + NADP+ Azospirillum brasilense
-
 L-arabinono-1,4-lactone + NADPH + H+
-
 ?
L-arabinose + NADP+ Azospirillum brasilense ATCC29145
-
 L-arabinono-1,4-lactone + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense Q53TZ2
-
-
Azospirillum brasilense ATCC29145 Q53TZ2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinose + NAD+
-
 Azospirillum brasilense L-arabinono-1,4-lactone + NADH + H+
-
 ?
L-arabinose + NAD+
-
 Azospirillum brasilense ATCC29145 L-arabinono-1,4-lactone + NADH + H+
-
 ?
L-arabinose + NADP+
-
 Azospirillum brasilense L-arabinono-1,4-lactone + NADPH + H+
-
 ?
L-arabinose + NADP+
-
 Azospirillum brasilense ATCC29145 L-arabinono-1,4-lactone + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
AraDH
-
 Azospirillum brasilense
NAD(P)-dependent L-arabinose 1-dehydrogenase
-
 Azospirillum brasilense

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Azospirillum brasilense

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
62.3
-
 NADP+ pH 9.0, 30°C Azospirillum brasilense
105.9
-
 NAD+ pH 9.0, 30°C Azospirillum brasilense

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Azospirillum brasilense

Cofactor

Cofactor Comment Organism Structure
NAD+ 570fold higher affinity with NADP+ (Km: 0.0028 mM) than NAD (Km: 1.586) when L-arabinose is used as a substrate. kcat value for NADP+ is 62.3/s, which is slightly smaller than that for NAD+ (105.9/s) Azospirillum brasilense
NADP+ 570fold higher affinity with NADP+ (Km: 0.0028 mM) than NAD (Km: 1.586) when L-arabinose is used as a substrate. kcat value for NADP+ is 62.3/s, which is slightly smaller than that for NAD+ (105.9/s) Azospirillum brasilense