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Literature summary for 1.1.1.376 extracted from
- D-galactose catabolism in archaea operation of the DeLey-Doudoroff pathway in Haloferax volcanii (2020), FEMS Microbiol. Lett., 367, fnaa029 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Haloferax volcanii H1209 | Haloferax volcanii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
NADP+ | pH 8.0, temperature not specified in the publication | Haloferax volcanii |  |
| 2 | 3 | D-galactose | pH 8.0, temperature not specified in the publication | Haloferax volcanii | |
| 35 | - |
L-arabinose | pH 8.0, temperature not specified in the publication | Haloferax volcanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | maximal activities are obtained at KCl concentrations higher than 1 M | Haloferax volcanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
- |
Haloferax volcanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + NADP+ | Haloferax volcanii | the enzyme has an essential function during growth on D-galactose | D-galactono-1,4-lactone + NADPH + H+ | - |
? | |
| D-galactose + NADP+ | Haloferax volcanii ATCC 29605 | the enzyme has an essential function during growth on D-galactose | D-galactono-1,4-lactone + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax volcanii | D4GR07 | - |
- |
| Haloferax volcanii ATCC 29605 | D4GR07 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloferax volcanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 323 | - |
substrate: D-galactose, pH 8.0, temperature not specified in the publication | Haloferax volcanii |
| 376 | - |
substrate: L-arabinose, pH 8.0, temperature not specified in the publication | Haloferax volcanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose + NAD+ + H2O | the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor | Haloferax volcanii | D-galactonate + NADH + H+ | - |
? | |
| D-galactose + NAD+ + H2O | the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor | Haloferax volcanii ATCC 29605 | D-galactonate + NADH + H+ | - |
? | |
| D-galactose + NADP+ | the enzyme has an essential function during growth on D-galactose | Haloferax volcanii | D-galactono-1,4-lactone + NADPH + H+ | - |
? | |
| D-galactose + NADP+ | the enzyme has an essential function during growth on D-galactose | Haloferax volcanii ATCC 29605 | D-galactono-1,4-lactone + NADPH + H+ | - |
? | |
| D-galactose + NADP+ + H2O | the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor | Haloferax volcanii | D-galactonate + NADPH + H+ | - |
? | |
| D-galactose + NADP+ + H2O | the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor | Haloferax volcanii ATCC 29605 | D-galactonate + NADPH + H+ | - |
? | |
| L-arabinose + NADP+ + H2O | - |
Haloferax volcanii | L-arabinonate + NADPH + H+ | - |
? | |
| L-arabinose + NADP+ + H2O | - |
Haloferax volcanii ATCC 29605 | L-arabinonate + NADPH + H+ | - |
? | |
| additional information | low activity was observed with D-xylose (5%), D-glucose, D-fructose and D-mannose are not oxidized at significant rates | Haloferax volcanii | ? | - |
- |
|
| additional information | low activity was observed with D-xylose (5%), D-glucose, D-fructose and D-mannose are not oxidized at significant rates | Haloferax volcanii ATCC 29605 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 39000, calculated from sequence | Haloferax volcanii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HVO_A0330 | - |
Haloferax volcanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Haloferax volcanii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor | Haloferax volcanii | |
| NADP+ | the catalytic efficiency for NAD+ is 230 times lower as for NADP+ indicating NADP+ to be the physiological electron acceptor | Haloferax volcanii | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | the deletion mutant does not grow on D-galactose | Haloferax volcanii |
| metabolism | the enzyme is part of the De Ley-Doudoroff pathway | Haloferax volcanii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7 | - |
L-arabinose | pH 8.0, temperature not specified in the publication | Haloferax volcanii | |
| 9.1 | - |
D-galactose | pH 8.0, temperature not specified in the publication | Haloferax volcanii | |
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