Literature summary for 1.1.1.376 extracted from

Aro-Kaerkkaeinen, N.; Toivari, M.; Maaheimo, H.; Ylilauri, M.; Pentikaeinen, O.T.; Andberg, M.; Oja, M.; Penttilae, M.; Wiebe, M.G.; Ruohonen, L.; Koivula, A.
L-arabinose/D-galactose 1-dehydrogenase of Rhizobium leguminosarum bv. trifolii characterised and applied for bioconversion of L-arabinose to L-arabonate with Saccharomyces cerevisiae (2014), Appl. Microbiol. Biotechnol., 98, 9653-9665.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae	Rhizobium leguminosarum bv. trifolii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.05	-	NADP+	L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
0.33	-	L-arabinose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
0.48	-	D-galactose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
0.5	-	D-galactose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
0.77	-	NAD+	L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
0.93	-	L-arabinose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
1.3	-	D-fucose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
2.72	-	D-fucose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
23.39	-	D-talose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
57.3	-	D-talose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
34800	-	-	Rhizobium leguminosarum bv. trifolii
67600	-	gel filtration	Rhizobium leguminosarum bv. trifolii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-arabinose + NAD+	Rhizobium leguminosarum bv. trifolii	-	L-arabinono-1,4-lactone + NADH + H+	-	?
L-arabinose + NADP+	Rhizobium leguminosarum bv. trifolii	-	L-arabinono-1,4-lactone + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Rhizobium leguminosarum bv. trifolii	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
StrepTactin Sepharose column chromatography	Rhizobium leguminosarum bv. trifolii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-fucose + NAD+	-	Rhizobium leguminosarum bv. trifolii	D-fucono-1,4-lactone + NADH + H+	-	r
D-fucose + NADP+	100% activity	Rhizobium leguminosarum bv. trifolii	?	-	?
D-fucose + NADP+	-	Rhizobium leguminosarum bv. trifolii	D-fucono-1,4-lactone + NADPH + H+	-	r
D-galactose + NAD+	-	Rhizobium leguminosarum bv. trifolii	D-galactono-1,4-lactone + NADH + H+	-	r
D-galactose + NADP+	69% activity compared to D-fucose	Rhizobium leguminosarum bv. trifolii	?	-	?
D-galactose + NADP+	-	Rhizobium leguminosarum bv. trifolii	D-galactono-1,4-lactone + NADPH + H+	-	r
D-talose + NAD+	-	Rhizobium leguminosarum bv. trifolii	D-talono-1,4-lactone + NADH + H+	-	r
D-talose + NADP+	12% activity compared to D-fucose	Rhizobium leguminosarum bv. trifolii	?	-	?
D-talose + NADP+	-	Rhizobium leguminosarum bv. trifolii	D-talono-1,4-lactone + NADPH + H+	-	r
L-arabinose + NAD+	-	Rhizobium leguminosarum bv. trifolii	L-arabinono-1,4-lactone + NADH + H+	-	?
L-arabinose + NADP+	-	Rhizobium leguminosarum bv. trifolii	L-arabinono-1,4-lactone + NADPH + H+	-	?
L-arabinose + NADP+	21% activity compared to D-fucose	Rhizobium leguminosarum bv. trifolii	L-arabinono-1,4-lactone + NADPH + H+	-	?
additional information	some minor activity (about 1%) is measured with D-xylose and L-lyxose, while no activity is detected with D-glucose or L-rhamnose	Rhizobium leguminosarum bv. trifolii	?	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 33526, calculated from amino acid sequence	Rhizobium leguminosarum bv. trifolii
homodimer	2 * 34800, SDS-PAGE	Rhizobium leguminosarum bv. trifolii

Synonyms

Synonyms	Comment	Organism
AraDH	-	Rhizobium leguminosarum bv. trifolii
L-arabinose/D-galactose 1-dehydrogenase	-	Rhizobium leguminosarum bv. trifolii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
20.85	-	L-arabinose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
35.45	-	NADP+	L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
38.12	-	D-galactose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
65.55	-	L-arabinose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
69.9	-	D-galactose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
74.58	-	D-talose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
85.73	-	NAD+	L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
99.52	-	D-talose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
110.1	-	D-fucose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
120.4	-	D-fucose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
9	-	-	Rhizobium leguminosarum bv. trifolii

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	the enzyme prefers NADP+ but uses also NAD+ as a cofactor	Rhizobium leguminosarum bv. trifolii
NADP+	the enzyme prefers NADP+ but uses also NAD+ as a cofactor	Rhizobium leguminosarum bv. trifolii

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.8	-	D-talose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
3.3	-	D-talose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
44.3	-	D-fucose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
63.5	-	L-arabinose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
70.7	-	L-arabinose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
80.2	-	D-galactose	with NAD+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
85.3	-	D-fucose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
111.8	-	NAD+	L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
140	-	D-galactose	with NADP+ as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii
741.7	-	NADP+	L-arabinose as cosubstrate, in 100 mM Tris-HCl, pH 9.0, 23°C	Rhizobium leguminosarum bv. trifolii

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Release 2023.1 (January 2023)