Literature summary for 1.1.1.374 extracted from

Namboori, S.C.; Graham, D.E.
Enzymatic analysis of uridine diphosphate N-acetyl-D-glucosamine (2008), Anal. Biochem., 381, 94-100.

Search for more literature for 1.1.1.374

Application

Application	Comment	Organism
analysis	high-throughput chromatographic analysis of UDPGlcNAc in a complex matrix of deproteinized cell extract	Methanococcus maripaludis

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli BL21(DE3)	Methanococcus maripaludis

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	N-acetyl-D-glucosamine, D-glucose, and D-glucosamine do not inhibit the reaction at 3-mM concentrations	Methanococcus maripaludis
UDP	2 mM, reduces activity by 50%	Methanococcus maripaludis
UDP-alpha-D-glucose	2 mM, reduces activity by 50%	Methanococcus maripaludis
UDP-alpha-N-acetyl-D-galactosamine	3 mM, reduces activity by 50%	Methanococcus maripaludis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.18	-	NAD+	pH 8.5, 37°C	Methanococcus maripaludis
0.18	-	UDP-N-acetyl-alpha-D-glucosamine	pH 8.5, 37°C	Methanococcus maripaludis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	activity is 85% lower in the absence of KCl as compared with reactions containing 200 mM KCl	Methanococcus maripaludis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	8 * 39000, SDS-PAGE	Methanococcus maripaludis
305000	-	gel filtration	Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-N-acetyl-alpha-D-glucosamine + NAD+	Methanococcus maripaludis	the enzyme is involved in the biosynthesis of glycan component sugars	UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanococcus maripaludis	Q6M0B9	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Methanococcus maripaludis

Storage Stability

Storage Stability	Organism
-80°C, stable for at least several weeks in 20% glycerol	Methanococcus maripaludis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-alpha-D-glucosamine + NAD+	the enzyme is involved in the biosynthesis of glycan component sugars	Methanococcus maripaludis	UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+	-	?
UDP-N-acetyl-alpha-D-glucosamine + NAD+	no activity is detected when NAD+ is replaced with NADP+. The MMP0352 protein does not catalyze the oxidation of the substrate analogs UDPGlc, UDPGalNAc, N-acetylglucosamine, and glucosamine	Methanococcus maripaludis	UDP-2-acetamido-3-dehydro-2-deoxy-alpha-D-glucopyranose + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
octamer	8 * 39000, SDS-PAGE	Methanococcus maripaludis

Synonyms

Synonyms	Comment	Organism
MMP0352	locus name	Methanococcus maripaludis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Methanococcus maripaludis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.9	-	UDP-N-acetyl-alpha-D-glucosamine	pH 8.5, 37°C	Methanococcus maripaludis
0.99	-	NAD+	pH 8.5, 37°C	Methanococcus maripaludis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	assay at	Methanococcus maripaludis

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in the biosynthesis of glycan component sugars	Methanococcus maripaludis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5	-	UDP-N-acetyl-alpha-D-glucosamine	pH 8.5, 37°C	Methanococcus maripaludis
5.5	-	NAD+	pH 8.5, 37°C	Methanococcus maripaludis

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Release 2023.1 (January 2023)