Cloned (Comment) | Organism |
---|---|
gene iolX | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an iolX disruption mutant strain BFS3018 from wild-type strain 168 | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
scyllo-inositol + NAD+ | Bacillus subtilis | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | - |
? | |
scyllo-inositol + NAD+ | Bacillus subtilis 168 | - |
2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P40332 | - |
- |
Bacillus subtilis 168 | P40332 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
scyllo-inositol + NAD+ | - |
Bacillus subtilis | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | - |
? | |
scyllo-inositol + NAD+ | - |
Bacillus subtilis 168 | 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DegA | - |
Bacillus subtilis |
IolX | - |
Bacillus subtilis |
NAD+-dependent SI dehydrogenase | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus subtilis | |
NADH | - |
Bacillus subtilis |
Organism | Comment | Expression |
---|---|---|
Bacillus subtilis | gene iolQ encodes a repressor of the iolX transcription, gene iolQ is organized in an operon immediately upstream of iolX, the repressor binds to the promoter region of iolX | down |
Bacillus subtilis | myo- and scyllo-inositol are intracellular inducers of gene iolX, but they fail to antagonize DNA binding of IolQ in in vitro experiments | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the inositol metabolic pathway in Bacillus subtilis, organization of the relevant genes, overview | Bacillus subtilis |
physiological function | Bacillus subtilis is able to utilize at least three inositol stereoisomers as carbon sources, myo-, scyllo-, and D-chiro-inositol (MI, SI, and DCI, respectively). NAD+-dependent SI dehydrogenase is responsible for SI catabolism. IolQ is a transcriptional repressor of iolX. Expression of gene iolQ is required to regulate gene iolX transcription in response to scyllo-inositol, identification of the two IolQ-binding sites within the iolX promoter region | Bacillus subtilis |