Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) CodonPlus cells and in Nicotiana benthamiana leaves | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
R162Q | inactive | Arabidopsis thaliana |
R162Q/R234Q | inactive | Arabidopsis thaliana |
R234Q | inactive | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Arabidopsis thaliana | 9536 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 0.1 mM used in assay conditions | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Arabidopsis thaliana | - |
oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | Arabidopsis thaliana | - |
(S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Arabidopsis thaliana | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Arabidopsis thaliana | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
L-malate-NAD-oxidoreductase | - |
Arabidopsis thaliana |
NAD-dependent MDH | - |
Arabidopsis thaliana |
pdNAD-MDH | - |
Arabidopsis thaliana |
plastidial NAD-dependent malate dehydrogenase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana | |
NADH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | Arabidopsis mutants lacking the enzyme are embryo-lethal, and constitutive silencing causes a pale, dwarfed phenotype | Arabidopsis thaliana |
physiological function | the enzyme is essential for early etioplast and chloroplast development due to its moonlighting role in stabilizing FtsH12, distinct from its enzymatic function | Arabidopsis thaliana |