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Literature summary for 1.1.1.37 extracted from

  • Demarsy, E.; Albertini, D.; Maier, B.A.; Kessler, F.; Hoertensteiner, S.; Zeeman, S.C.; Koetting, O.
    Plastidial NAD-dependent malate dehydrogenase A moonlighting protein involved in early chloroplast development through its interaction with an FtsH12-FtsHi protease complex (2018), Plant Cell, 30, 1745-1769 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) CodonPlus cells and in Nicotiana benthamiana leaves Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
R162Q inactive Arabidopsis thaliana
R162Q/R234Q inactive Arabidopsis thaliana
R234Q inactive Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
plastid
-
Arabidopsis thaliana 9536
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 0.1 mM used in assay conditions Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Arabidopsis thaliana
-
oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH + H+ Arabidopsis thaliana
-
(S)-malate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Arabidopsis thaliana oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH + H+
-
Arabidopsis thaliana (S)-malate + NAD+
-
r

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
L-malate-NAD-oxidoreductase
-
Arabidopsis thaliana
NAD-dependent MDH
-
Arabidopsis thaliana
pdNAD-MDH
-
Arabidopsis thaliana
plastidial NAD-dependent malate dehydrogenase
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction Arabidopsis mutants lacking the enzyme are embryo-lethal, and constitutive silencing causes a pale, dwarfed phenotype Arabidopsis thaliana
physiological function the enzyme is essential for early etioplast and chloroplast development due to its moonlighting role in stabilizing FtsH12, distinct from its enzymatic function Arabidopsis thaliana